The Research Of ShenQi Compound Recipe On Differential Gene Expression In KKAy Mice With Diabetic Macroangiopathy

Objective: Through observing the effect of ShenQi Compound Recipe, which has the function of supplementing Qi and Yin, and promoting the blood circulation, on the aortic pathological morphology of KKAy mice with diabetic vascular disease, and finding the molecular targets of prevention and treatment of diabetic macroangiopathies, by using the gene chip technology, we explored the biological effects and mechanisms, and providing the molecular basis of ShenQi Compound Recipe in preventing diabetic macroangiopathies.Methods:66male KKAy mice,18-20weeks old, random blood glucose>13.9mmol/l, were divided to four groups:the KKAy group, diabetic macrovascular disease group, the ShenQi Compuound Prescription group, and rosiglitazone group, each group had15mice.15Normal C57BL/6J mice were set as normal group. The model group, ShenQi Compuound Prescription group and rosiglitazone group mice were induced by adding The Nco-nitrio-L-arginine methyl ester (L-NAME) with0.2mg. ml-1.d-1into the drinking water for8weeks. The normal group were fed with normal diet, while others with high fat diat. At the same time, ShenQi Compuound Prescription were administered ShenQi compound recipe with14.4g·kg-1·d-1, and rosiglitazone group was administered rosiglitazone suspension with1.33mg. kg-1.d-1, while others were administered physiological saline with10ml. kg-1.d-1. In the experimental period, general status were observed as well as the amount of water drinking, food intake, body weight, and random blood glucose. After8weeks, the mice were sacrificed to detect the fasting blood glucose, insulin, lipid levels, HE Staining in abdominal aortic for observing aortic morphology, detecting the gene expression profiles of the Thoracic aorta by microarray gene protein technique, Screening the differentially expressed genes, GO annotations, Pathway analysis, and explaining the biological significance.Results:the general state of ShenQi Compound Recipe group were better than that of model group, with drinking less, body weight being decreased, as well as blood glucose, blood lipids. The Differentially expressed genes of ShenQi Compound Recipe group,compared with the model group, through GO annotation, pathway analysis, mainly involved in cell fate commitment, localization of cell, and macromolecule metabolic processes, as well as the Insulin signaling pathway, Adipocytokine signaling pathway and Cysteine and methionine metabolism, and the gene involved were Kdr、FGFR4、Met、AMPK.Conclusion: ShenQi Compound Recipes can improve the general state of KKAy mice with diabetic macrovascular disease, reducing the amount of drinking water of the model mice, reducing the body weight, improving lipid metabolism disorders, alleviating the vascular endothelial injury, delaying the progress of atherosclerosis. The mechanism may be repairing the damaged endothelial cells in the early stage of diabetic macrovascular disease, reducing the damage to blood vesssel of Homocysteine, Metabolites of Cysteine and methionine, through regulating cell fate commitment, localization of cell, macromolecule metabolism, as well as the Insulin signaling pathway, Adipocytokine signaling pathway,Cysteine and methionine metabolic signaling, as well as genes such as Kdr、FGFR4、Met、AMPK.