The Research Of Early Use Of Blood Activating In ShenQi Compound Recipe On Differential MicroRNAs Expression In KKAy Mice With Diabetic Macroangiopathy

Objective:The present study is to determine the effects of the early use of blood components in Shenqi compound recipe on the macroangiopathy in spontaneously diabetic KKAy mice,Purporting from overall level, cellular level and molecular level in the preliminary discussion of ginseng and astragalus compound compatibility of blood component rationality and scientific nature,inorder to provide theoretical basis and mechanism research for early use in preventing and curing diabetic vascular lesions.Methods:50 male KKAy mice,7-8 weeks old, random blood glucose≥ 13.9mmol/l, were divided to five groups:the bland group, the ShenQi Compuound Prescription group,the blood group, atorvastatin group and the diabetic macrovascular disease group,each group had 10 mice. The model group, ShenQi Compuound Prescription group,the blood group and simvastatin group mice were induced by adding The Nω-nitrio-L-arginine methyl ester (L-NAME) with 0.2mg. ml-1.d-1 into the drinking water for 5 weeks. The blank group were fed with normal diet,while others with high fat diat. At the same time, ShenQi Compuound Prescription and the blood components were administered ShenQi compound recipe with 14.4g·kg-1·d-1, and simvastatin group was administered atorvastatin suspension with 1.33mg. kg-1.d-1, while others were administered physiological saline with 10ml. kg-1.d-1 In the experimental period, general status were observed as well as the amount of water drinking, food intake, body weight, and random blood glucose. After 5 weeks, the mice were sacrificed to detect the fasting blood glucose, lipid levels,hs-crp levels, HE Staining in abdominal aortic for observing aortic morphology, application of Agilent micrornas chip technology platform to test the miRNA expression in aortic tissue,screening differentially expressed miRNAs,And predicting its target genes using TargetScan databases, GO annotations further on the target genes and Pathway of bioinformatics analysis, with a deep understanding of the regulation of miRNAs mechanism and biological function.Results:The general state of ShenQi Compound Recipe group and the blood group were better than that of model group, with body weight being decreased, as well as blood glucose, blood lipids,hs-crp. The Differentially expressed miRNAs of blood group,compared with the model group, After the database predicted target genes,through GO annotation, pathway analysis, mainlyinvolved in cell fate determination, improve the stress response and cell adhesion 3 biological processes and regulation of the actin cytoskeleton regulation, epidermal growth factor signaling pathway, p38MAPK signal through the relevant.Conclusion:Both of the shenQi Compound Recipes and its blood components can improve the general state of KKAy mice with diabetic macrovascular disease, reducing the amount of drinking water of the model mice, reducing the body weight, improving lipid metabolism disorders, alleviating the vascular endothelial injury, delaying the progress of atherosclerosis. The mechanism may be repairing the damaged endothelial cells in the early stage of diabetic macrovascular disease, reducing the damage to blood vesssel of Homocysteine, Metabolites of Cysteine.Its mechanism may be through regulation of biological process in cell fate determination, improve the stress response and cell adhesion and actin cytoskeleton regulation, epidermal growth factor signaling, p38MAPK signaling, and the prevention of diabetic macroangiopathy.