| Objective:Observe the influence on banxiaxiexin decoction and diffenent herbal formulation of component about gastric ulcer rat model in respects of inflammatory factors, repair factor and Hsp27, Hsp70protein expression, and analysis the mechanism of action about the treatment effects of banxiaxiexin decoction and its Subdivisions, investigate the Significance of diffenent herbal formulation of component in banxiaxiexin decoction on treat gastric ulcer (GU), reveal the superiority and targets of compatibility of both cold and heat.Methods:Select male and female SD rats and establish the gastric ulcer animal model of mixed cold and heat syndromes. After24hours, all the rats except blank group and sham operation group were given of banxiaxiexin decoction and different herbal formulation of component, all rats were sacrificed after28days, observe rats" pathological morphology change of gastric tissue, test SOD activity in xanthine oxidase method, gastric tissue MDA content in thiobarbituric acid method, serum NO Content in nitrate reductase method, gastric tissue TNF-a, IL-8, GAS, MTL and serum PAF, ET content in ELISA method, gastric mucosal EGF, VEGF, bFGF, PDGF expression in immunohistochemical detection, HSP27, HSP70mRNA expressions of gastric tissue of rats in RT-PCR method.Result:①General behavior:The rats’spirit, diet, fur color and weight were signify-cantly improved by banxiaxiexin decoction group and compatibility of both cold and heat group;②Pathoio-gical observation:The blank group and sham operation group rats’ structure of gastric mucosa surface was complete, and there were no congestion, edema, the simple columnar epithelial cells were arranged in neat rows, basically no defect, shedding no inflammatory cell infiltration in the lamina propria, glands regular shape;The model group rats’structure of gastric mucosa surface was thinning, Focal epithelial cell shedding, the defect in the lamina propria massive infiltration of inflammatory cells, part of the formation of lymphoid follicles, lamina propria glands significantly reduce sparse arrangement disorder; After treatment, Significantly reduced banxiaxiexin decoction group and compatibility of both cold and heat group,these pathological changes, the basic integrity of the structure of gastric mucosa epithelial structure repair, a large number of lamina propria gland hyperplasia, arranged in neat rows, cystic expansion, serosal integrity, only to see a small amount of inflammatory cellsinfiltration; the rest of the group is still visible inflammatory cell infiltration and inflammation;③TNF-α、IL-8content of gastric tissue:Compared with blank group, the content ofTNF-α、IL-8were significantly elevated of model group rats (P<0.01); Compared with model group, the content of TNF-α、IL-8were significantly decreased of banxiaxiexin decoction group, compatibility of both cold and heat group, cold group and positive group (P<0.01); Compared with banxiaxiexin decoction group, the content ofTNF-α、IL-8were no significantly differenee(P>0.05);④SOD、MDA content of gastric tissue:Compared with blank group, the content ofSOD was significantly decreased of model group rats(P<0.01), the content of MDA were significantly elevated of model group rats (P<0.01); Compared with model group, the content of SOD was significantly elevated of banxiaxiexin decoction group, compatibility of both cold and heat group, heat group, tonic group, heat tonic group and cold tonic group (P<0.01),while the content of MDA was significantly decreased (P<0.01); Compared with banxiaxiexin decoction group, the content of SOD was significant difference of cold group (P<0.01),while it was difference of tonic group and cold tonic group (P<0.05), the content of MDA was significant difference of cold group, cold tonic group and positive group (P<0.01), while it was difference of tonic group(P<0.05);⑤GAS,MTL content of gastric tissue:Compared with blank group, the content of GAS,MTL were significantly elevated of model group rats (P<0.01); Compared with model group, the content of GAS,MTL were significantly decreased of banxiaxiexin decoction group, compatibility of both cold and heat group, cold tonic group and positive group (P<0.01); Compared with banxiaxiexin decoction group, the content of GAS was significantly decreased of compatibility of both cold and heat group, positive group (P>0.05), the content of MTL was significantly decreased of compatibility of both cold and heat group, cold group and cold tonic group (P>0.05).⑥PAF、ET、NO content of serum:Compared with blank group, the content of PAF、ET of serum were significantly elevated of model group rats while the content of NO of serum decreased obviously (P<0.01); Compared with model group, the content of PAF、ET of serum were significantly decreased of banxiaxiexin decoction group, compatibility of both cold and heat group, heat group, heat tonic group (P<0.01) while it was decreased of cold group and cold tonic group (P<0.05), the content of ET of serum were significantly decreased of tonic group and cold tonic group (P<0.05), the content of ET of serum were significantly elevated of banxiaxiexin decoction group, compatibility of both cold and heat group, heat group, tonic group,heat tonic group, cold tonic group and positive group (P<0.01); Compared with banxiaxiexin decoction group, the content of PAF of compatibility of both cold and heat group was no significant difference (P>0.05), the content of ET of compatibility of both cold and heat, heat group were no significant difference (P>0.05), the content of NO of compatibility of both cold and heat, heat group and heat tonic group were no significant difference (P>0.05);⑦Gastric mucosal EGF, VEGF, bFGF, PDGF expression:Compared with blank group, the average optical density of EGF, VEGF, bFGF, PDGF expression were significantly enhanced of model group rats(P<0.01); Compared with model group,the average optical density of EGF, VEGF expression were significantly enhanced of banxiaxiexin decoction group, compatibility of both cold and heat group, tonic group, heat tonic group, cold tonic group and positive group (P<0.01), while it was enhanced of heat group (P<0.05), cold group was no significant difference (P>0.05), the average optical density of bFGF, PDGF expression were significantly enhanced of banxiaxiexin decoction group, compatibility of both cold and heat group, tonic group, heat tonic group, cold tonic group and positive group (P<0.01), cold group and heat group was no significant difference (P>0.05); Compared with banxiaxiexin decoction group, compatibility of both cold and heat group, tonic group, heat tonic group, cold tonic group were no significant difference (P>0.05), cold group and heat group were significant difference (P<0.01).⑧HSP27, HSP70mRNA expression of gastric tissue: Compared with blank group, the HSP27, HSP70mRNA expression of gastric tissue were significantly enhanced of model group rats (P<0.01); Compared with model group, the HSP27, HSP70mRNA expression of gastric tissue were significantly enhanced of banxiaxiexin decoction group, compatibility of both cold and heat group, Heat tonic group (P<0.01), while it is enhanced of Heat group, tonic group, Cold tonic group and positive group (P<0.05), cold group was no significant difference (P>0.05); Compared with banxiaxiexin decoction group, compatibility of both cold and heat group was no difference (P>0.05).Conclusion:①Banxiaxiexin decoction and diffenent herbal formulation of component can improve the gastric ulcer rat’s General behavior and reduce the inflammation degree of rats" gastric ulcer significantly.②Banxia(?)exin decoction and diffenent herbal formulation of component have obvious therapeutic effect on gastric ulcer. The mechanisms of action include inhibition of inflammatory factors release and improve the body’s antioxidant capacity and mitigate the damage of lipid peroxidation, alleviate gastric acid erosion of the gastric mucosa, coordination of gastrointestinal motility, improve microcirculation, improve gastric mucosal’s ability to repair, upregulation of endogenous protective protein HSP27, HSP70mRNA expression.③The different properties drug of banxiaxiexin decoction plays a therapeutic role from different aspects on gastric ulcer. Cold group of drugs play an important part on reducing the TNF-a, IL-8, GAS and MTL content of gastric tissue; Heat group of drugs play an important part on increasing the NO content of serum,reducing the PAF, ET content of serum, Tonic group of drugs play an important part on increasing the EGF, VEGF, bFGF and PDGF positive expression in gastric mucosa; Heat tonic group of drugs play an important part on increasing the SOD content of gastric tissue and reducing the MDA content of gastric tissue, upregulation of endogenous protective protein HSP27, HSP70mRNA expression; Cold tonic group of drugs play an important part on reducing the GAS, MTL content of gastric tissue;④After compatibility of both cold and heat drugs, the synergistic effect significant was reinforced. Compatibility of cold and heat drugs can reduce the limitations and deficiencies of cold or heat drugs and embody the Chinese medicine targeted therapeutic advantage. |
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