Effect Of Dajianzhong Decoction And Decomposed Formulas On Spleen And Stomach Deficiency-cold Gastric Ulcer Model Rats

Objective:Through the experiment,observe whether Dajianzhong decoction and its decomposed formulas have the effect of anti-spleen-stomach deficiency cold restraint-water immersion stress gastric ulcer.Aiming to explore the mechanism of the prescription and reveal the results of different drug combinations,then providing new ideas and methods for the research of Dajianzhong decoctionMethods:60 SPF SD rats were divided into 6 groups.They were the blank control group,the model control group,the omeprazole positive drug group,the Dajianzhong decoction group,the tonify deficiency group(ginseng,maltose),and the dispel pathogenic group(pepper,dried ginger),10 rats in each group.The blank control group was routinely fed,and the rats in the other groups were established the model of spleen-stomach deficiency cold by rhubarb-diarrhea method combined with diet disorder method.After the success of the model,in addition to the blank control group and the model control group,each treatment group was given prophylactic drugs,the positive drug group was given the omeprazole suspension solution,and the other groups were given the corresponding dose of medicine decoction for treatment rats,2 times per day for 7 consecutive days.After given drugs,the rat model of stress ulcer was established by the method of restraint-water immersion,and then the brain tissue,stomach tissue and serum of rats were taken and tested by ELISA method.To measure the content of pepsin,SOD and MDA in gastric tissue,the content of 5-HT in brain tissue,the content change of NO and PGE2 in serum.Histopathological changes of stomach observed by light microscope.SPSS19.0 statistical software was used for data analysis and processingResults:1.In the process of establishing the model of deficiency cold of spleen and stomach,the rats were lethargic,depressed,clumped up,sluggish in action,chilly and squinting,weight loss,loose stools,withered hair and so on Combined with the macroscopic symptom scores of deficiencies cold of spleen and stomach,the rats were all above 5 points(P<0.01),indicating that the model of deficiency cold of spleen and stomach was successful2.Pathomorphological observation:compared with the blank group,the epithelial cells of gastric mucosa in the model group were significantly exfoliated,the submucosa was obviously congested and edematous,and a large number of inflammatory cells were infiltrated.Compared with the model group,the pathological state of each treatment group was relieved,and the effect of Dajianzhong decoction group is the best3.UI,pepsin content:compared with the model group,the ulcer index and pepsin content in each treatment group were significantly lower(P<0.01);compared with Dajianzhong decoction group,there was significant difference(P<0.01)in the tonify deficiency group and dispel pathogenic group,There was no difference in positive drugs(P>0.05)4.NO,PGE2 content:compared with the model group,the NO content in the Dajianzhong decoction group,the positive medicine group and the deficiency tonifying group increased significantly(P<0.01),but there was no difference in the dispel pathogenic group(P>0.05),and the PGE2 content in each treatment group increased significantly(P<0.01);compared with the Dajianzhong decoction group,the NO and PGE2 content in the dispel pathogenic group were significantly different(P<0.01),but there was no difference in the positive medicine group and the deficiency tonifying group(P>0.05)5.SOD,MDA content:compared with the model group,SOD content in each treatment group was significantly higher,with significant difference(P<0.01),MDA content in the Dajianzhong decoction group,positive control group and tonify deficiency group were significantly lower,with significant difference(P<0.01);there was difference in the dispel pathogenic group(P<0.05);Compared with the Dajianzhong decoction group,the content of SOD and MDA in the positive drug group,tonify deficiency group and dispel pathogenic group were significantly different(P<0.01)6.5-HT content:compared with the model group,the 5-HT content of the Dajianzhong decoction group and the tonify deficiency group was significantly lower,with a significant difference(P<0.01),the positive medicine group and the dispel pathogenic group were significantly different(P<0.05);compared with the Dajianzhong decoction group,the positive medicine,the tonify deficiency group and the dispel pathogenic group were significantly different(P<0.01)Conclusion:1.Dajianzhong decoction and its decomposed formulas have the prevention and treatment effect on the restraint-water immersion stress gastric ulcer of deficiency-cold of spleen and stomach,and the prevention and treatment effect of Dajianzhong decoction group is the best2.After Dajianzhong decoction was disassembled,tonify deficiency group and dispel pathogenic group had different degrees of prevention and treatment effect on the restraint-water immersion stress gastric ulcer of deficiency-cold of spleen and stomach,and the effect of tonify deficiency group was better than dispel pathogenic group3.The mechanism of Dajianzhong decoction on the restraint-water immersion stress gastric ulcer of deficiency-cold of spleen and stomach may be related to the increase of SOD,NO,PGE2,the decrease of MDA and 5-HT.