Study On The Clinical Basis For Treatment Of Acute Leukemia By Tonicing Qi And Nurishing Yin Decoction And Its Decomposed Recipes Based On Data Mining And Experimental Studies Of Its Mechanism Of Inducing KG1a Cell Apoptosis

Objective Study on the traditional Chinese medicine syndrome and herbaladministration regularity of acute leukemia based on Data Mining, in order to offer theevidence-based data supporting to guide the TCM differential treatment of diabetic arterialobliterations. KG1a/NOD-SCID mice was used as the study platform,to explore themechanism of leukemia cell apoptosis induced by Tonicing Qi and Nurishing YinDecoction and its decomposed recipes.Methods The research literature were collected from periodical database withinrecent30years, focus on Chinese medicine syndrome and the medicine that had been used.Database was established with Excel software.To find the common syndrome, influencefactors,the commonly used medicines and medicine category by using frequency analysis.To obtaine information from the TCM four diagnostic methods by using cluster analysis.Toexploer Compatibility Law of herb by using association analysis.Taking the NOD-SCID mice as the experimental object,and injected with KG1a cellsfrom the tail vein after Irradiation by60Co.Model mices were randomly divided into modelgroup,Fuzheng group,Quxie group,Quanfang group.flow cytometry was applied tomeasure the cell cycle and apoptosis.The immunochemistry method was used to detect theexpression of c-myc,survivin,p21in mices’ lymph node tissue.The PCR-ELISA wasapplied to measure the Telomerase Activity of bone marrow cells.The expression ofhTERT mRNA and methylation of its promoter were measured using RT-PCR. Results1. The common syndrome of acute leukemia are deficiency of both qi and yin, heattoxin syndrome, deficiency of both qi and blood, and junction of sputum,thermal andstasis.Influence factors include thermal,qi deficiency and yin deficiency.The target sites ofinfluence factors are kidney,liver and spleen.2. The commonly used medicine categorise are tonifying herbs,heat-clearingherbs,blood-activating and stasis-resolving herbs, removing dampness and promotingdiuresis herbs,apophlegmatisant herbs.Tonifying herbs and heat-clearing herbs occupy animportant position.The most common Flavors of Drugs are Sweet and bitter.Cold drug inall drug occupies the main position.Secondly it is warm drug,and the next is ping drug.Thechannel-tropism of medicine is mostly liver,secondly lung,spleen and kidney.3. After treatment,survival time of all treatment groups were longer than those inmodel group（P<0.05）. Apoptosis rate and the proportion of cells in the G1phase in alltreatment groups were increased（P<0.05）. The expression of c-myc、survivin in mices’lymph node tissues as well as hTERT mRNA and methylation of its promoter,and theTelomerase Activity of bone marrow cells in all treatment groups were significantlydeclined compared with model group（P<0.05）.Howere, the expression of p21in mices’lymph node tissues in all treatment groups was higher than model group（P<0.05）.TheEffect of Quanfang group on the all Indices was superior to Fuzheng group and Quxiegroup（P<0.05）.Conclusion Through studing on the traditional Chinese medicine syndrome andherbal administration Rregularity of acute leukemia based on Data Mining, we obtained acorollary that the basic pathogenesis of acute leukemia was root deficiency and branchexcess.Root deficiency were deficiency of both qi and yin, deficiency of both qi andblood.Branch excess were heat toxin,sputum and stasis.On the treatment,not only ispel evilbut assist the justice.Supplementing qi and nourishing yin, heat-clearing and detoxifyingare the main treatment methods,and combined with water dampness, promoting bloodcirculation and removing blood stasis methods.Experimental studies proved that Tonicing Qi and Nurishing Yin Decoction inducedleukemia cell apoptosis,regulatid cell cycles,declined the expression of hTERT mRNA andmethylation of its promoter,effected the telomerase activity, Induce apoptosis,inhibit theproliferation of leukemia cells,prolonged the survival time of KG1a/NOD-SCID mices.So,Tonicing Qi and Nurishing Yin Decoction has significant therapeutic action on acuteleukemia.