The Research Of Chronic Skin Ulcer Treated By Cockroach Extract Liquid

Objective:chronic skin ulcer is a common. refractory disease with long course.it makes patients suffer great physical and psychotogical pain.At present, its difficult to heal the chronic skin ulcer,and the healing mechanism is not completely clear. This research aims to explore the mechanism of healing of chronic ulcer of skin from the level of molecular biology by laboratory and clinical research on the chronic skin ulcer.To further explore the working target of Chinese medicine treatm-ent, meanwhile,to confirm the effectiveness of cockroachextract liquid in treatment of chronic skin ulcer with objective data. The ultimate aim is to provide laboratory basis and data support for seeking the effective、convenient and inexpensive treatment of chronic skin ulcer.Methods:68sufferers who meet the inclusion criteria of chronic skin ulcer were randomly divided into experimental group and control group. the Infected patients should be given anti-infection treatment, the patients with diabetesmellit-us can also take hypoglycemic agents. clean the ulcer with physiological saline cotton, then wipe the ulcer with dry cotton, the experimental group sufferers were treated with cockroach extract liquid wet compress(Kangfuxin liquid),while spread the Shengjisan over the ulcer surface of control group sufferers, finally bandaged ulcer the with sterile gauze. The two groups were changed dressing everyday, for4weeks. To observe the healing course and cure rate. Before and after the treatment, took the skin lesions tissue samples of the two groups,the expression of Smad3gene、TGF-β1and VEGF was examined by immunohistochemistry method.Results:Before treatment, the skin lesion area、the score of Secretion、the score of skin lesions clour、the score of skin lesions odor、the score of soreness and asting plasma glucose level of both experimental group and control group were comparable,with no significant difference (P>0.05);After1,2,3,4weeks of treatment, the skin lesion area、the score of secretion、the score of skin lesions clour of both experimental group and control group were comparable,with no significant difference (P>0.05);After1,2weeks of treatment, the score of skin lesions clour of both experimental group and control group were comparable,with no significant difference(P>0.05), After3,4weeks of treatment, the score of skin lesions clour of experimental group were significantly lower than the control group date(P<0.05);t After1,2,3weeks of treatment, the score of soreness of both experimental group and control group were comparable,with no significant difference(P>0.05), After4weeks of treatment, the score of soreness of experimental group were obviously lower than the control group date (P<0.05);the cure rate. success rate、better rate and the rate of Inefficiency was8.82%、29.41%、47.06%and14.71%respectively；while the date for the control group was14.71%、26.47%、47.06%and11.76%respectively; for experimental group,the number of patients whose asting plasma glucose level below6.11umol/ml、between6.12and11.1umol/ml、 above11.2umol/ml was21、8and5,while the result of control group was23、6and5;for experimental group;the number of patients who had suffered bedridden or disabled in action、diabetes、varicose vein of lower limb、skin injure or radiation was3、13、3、7、21、2, while the number of control group patients was4、11、18、252; because of the cockroach extract liquid wet compress (Kangfuxin liquid),the patients who suffered pain or itching was1and2,because of theShengjisan,the patients who suffered pain or itching was2and2;for the patients of both experimental group and control group,about60%of them was between41～50years old and between51～60years old.Before treatment, for the experimental group,the percentage of Smad3gene、TGF-β1and VEGF was79.62±5.26%、29.74±3.12%、21.65±3.12%; for the control group, the the percentage of Smad3gene、TGF-β1and VEGF expressed positive cells was78.32±4.97%、29.13±3.98%、22.44±3.11%、 After4weeks of treatment, for the experimental group,the Smad3gene、TGF-β1and VEGF was18.85±1.88%、79.07±7.80%、59.24±3.74.%；for the control group, the date was20.03±2.42%,77.47±7.68%、61.09±4.10%. Before treatment,the expressed positive cells of Smad3gene、TGF-β1and VEGF in both experimental group and control group were comparable,with no significant difference (P>0.05);after the treatment for4weeks,the expressed positive cells of Smad3gene in experimental group is fewer than the control group obviously (P<0.05),while the TGF-β1and VEGF were more than control group obviously (P<0.05);when Completed the experiment,the expressed positive cells of Smad3gene reduce, and the expressed positive cells of TGF-β1and VEGF increased obviously (P<0.05).Conclusion:1. cockroach extract liquid can promote wound healing, clinical curative effect of cockroach extract liquid was similar to that of control group; after treatment, the skin lesion area、the score of Secretion、the score of skin lesions odor、success rate、better rate and the rate of Inefficiency of both experimental group and control group has no obvious difference; three indicators of clinical curative effect including the score of skin lesions soreness. the score of skin lesions clour and the cure rate of experimental group is better than the control group; the asting plasma glucose level and medical history analysis of the two groups has no obvious difference;3cases in experimental group and4cases in control group complainof pain or itching after treatment;In cases of the experimental group and control group, the cases who were41to50years old and up to51-60years old were accounted for about60%of the total. The treatment of cockroach extract liquid for chronic skin ulcer is effective, safe.2.cockroach extract liquid probably inhibit the expression of Smad3gene, meanwhile,promote the TGF-β1, VEGF generation and migration, promote newborn of ulcer granulation tissue and regeneration of capillary, thus speed up the chronic skin ulcer healing.