Research On The Immunological Effects Of Tai Chi

Objective:The purpose of this dissertation is to study the effect of Tai chi on fitness byanalysis the change of immune system following Tai chi movements. The immunesystem index which used in this study contains T lymphocyte subsets, natural killercells (NK), natural killer T cells (NKT), dendritic cells (DC), Th1/Th2cell subsets ofrelated cytokines.Methods:Longitudinal study:170(76males,94females) healthy subjects aged55-65yrs, who volunteered to participate in this study by recruiting in Shanghai and wererandom divided into control group(38males,47females) and experimental group(38males,47females). The subjects were trained24weeks (60minutes per time,3timesper week) using Tai chi breathing exercise,24movements and42movements Tai chiin experimental group and without any tai chi movement in control group.Horizontalexperimental:60（control group30， experimental group30）female subjects aged55-65yrs. Experimental group who volunteered to participate in this study byrecruiting in Yang style Tai Chi Association and Chen Style Tai chi Association andtrained by brisk walking in Shanghai. The exercise period of subjects was3-5yrs(more than one hour per time, more than3times per week) in experimental group.Thesubjects’ cubital vein bloods were taken at rest on the morning of the day before andafter experiment in longitudinal study. The time of taking blood was the same inhorizontal experimental as the second time of taking blood in longitudinal study. Theexpression of T lymphocyte subsets (CD3+, of CD4+and CD8+), NK cells, NKT cellsand in vitro amplification secretion of cytokines (IFN-γ, IL-4), complement regulatoryproteins CD55and CD59were analysis using flow cytometry and ELISAenzyme-linked immunosorbent and so on.Results:1. Compared to baseline, the expression of CD3+, CD4+T lymphocytes, CD4+/CD8+ratio and NK cells were increased following Tai chi movements after24weeks. Thepercentage content of NK cells was the highest in Yang style Taichi group than others.2. Compared to baseline, the number and the activity of NKT cells was significantly increased following Tai chi movements after24weeks; The number of NKT cells wasthe highest in Chren style Taichi group than others; The activity of NKT cells washighest in Yang style Taichi group than others.3. Both IFN-γ and IL-4were increased, especially IFN-γ, were increased followingTai chi movements after24weeks; IFN-γ was lower in control group thanexperimental group. IL-4was higher in Yang style Taichi movement group thancontrol group.4. Compared to rest, lymphocytes, neutrophils, monocytes and red blood cell surfaceCD55mean fluorescence intensity were reduced after24movement Tai chi; there is asignificant reduction on monocytes and red blood cell surface CD55; compared torest, there was a significant reduction in monocytes and red blood cell surface CD59after24movements Tai chi; compared to rest, lymphocytes and monocytes andcell surface CD55mean fluorescence intensity was significant higher after tai chimovement; The red blood cell surface CD55was significant reduce and thelymphocytes cell surface CD59was significant increase after exercise.5. Lymphocytes, neutrophils, monocytes and red blood cell surface CD55meanfluorescence intensity were lower in.Yang style Taichi, Chen style Taichi and briskwalking group than control group; The CD59mean fluorescence intensity washigher in experimental group than control group; Yang style Taichi group lymphocytesurface CD59was significantly higher than other groups; groupYang style Taichi,Chen style Taichi group had red blood cell surface CD59was significantly higher thanthe brisk walking group and the control group.6. DC123+change was not statistically significant, but CD11c+was significantlyincreased after.24weeks of Taichi; Yang style Tai Chi, Chen style Taichi, and briskwalking group subjects CD11c+, CD123+higher than the control; Chen style Taichigroup CD11c+was the highest.Conclusions:1. The ability of anti-tumor and chronic infection can been improved by improving Tlymphocytes and NK cell numbers, effectively and the immune function of the elderly.Compared to Yang style tai chi and brisk walking, The CD3+was higher in long-termChen style tai chi exercisers. These results suggested that exercise intensity is the totalnumber of T lymphocytes. 2. Tai chi can increase the number and activity of NKT cells in the peripheral blood ofthe elderly as the movement in stimulation environmental factors, and effectivelyimprove the immune balance to Th2direction of drift in the process of immunesenescence, and play a positive role in maintaining the immune balance.3. Tai chi can activate the negative feedback mechanism regulated of the elderly, butthere were different influence in the white blood cell subsets (lymphocytes, neutrophils,monocytes) and red blood cell surface of CD55, CD59, the influence of which the redblood cells more obvious. This can provide theoretical support for the further study ofthe relationship between red blood cell immunity and exercise of the complementsystem.4. Tai chi movement can increase the number DC in peripheral blood in the elderly,the change of dendritic cells of myeloid CD11c+was more sensitive.They can be usedas target cells for further study of Tai chi on the immune function of the regulatorymechanisms.5. Yang style tai chi and Chen style tai chi on the positive impact of NK cells, NKTcells and red blood cell surface complement regulatory proteins significantly betterthan brisk walking group and control group. Maybe this is related with uniquemovement of tai chi the way.6. Tai chi as a traditional exercise and take care of the way, Spirit Heart, Energy aneffort to approximate constitute a neuroendocrine-immune regulation network, play animportant role in the process of mediated “immune senescence”...