TLR4Signaling Pathway Cytokines Differential Expression In Rats With Liver Injury Induced By Obstructive Jaundice After Intervention

Objective:To observe the effects of medium-intensity aerobic exercise in combination with bone marrow mesenchymal stem cells (BMSCs) or major bupleurum decoction (MBD) on the inflammatory cytokines of liver in rats with obstructive jaundice.Methods:Forty healthy male SD rats, weighing180-250g, were randomly divided into normal group (N, n=10), obstructive jaundice group (OJ, n=10), BMSCs plus exercise group (BMSCs+E, n=10) and MBD plus exercise group (MBD+E, n=10). The obstructive jaundice model was established by bile duct hang. BMSCs+E group rats were administered0.5ml BMSCs via the portal vein, MBD+E group rats were orally administered with MBD for12weeks, BMSCs+E group and MBD+E group with academic training, a total of training for12weeks. All the rats were sampled at the appointed time and various target markers were determined as follows:pathological changes were observed by microscope; expression of TLR4and MyD88proteins were detected with SABC; TNF-a, IL-6and IL-10mRNA expression were determined with RT-PCR; ALT, AST and TBIL were also measured.Results:Liver cable arranged normally in N group. Fibrosis, degeneration and necrosis in liver cell appeared in OJ group. Disorder of liver cable structure and light necrosis were seen and ALT, AST and TBIL reduced in MBD+E group (P<0.05). Insignificant necrosis, significant reduction in ALT, AST and TBIL and basically normal lobular architecture were observed in BMSCs+E group as compared with those in OJ group (P<0.01). Expression of TLR4and MyD88were significantly reduced in BMSCs+E group and MBD+E group as compared with those in OJ group. The expression of inflammatory mediators such as TNF-a and IL-6in BMSCs+E group was lower than OJ group (P<0.01), while IL-10level was significantly higher than that of OJ group (P<0.01)Conclusions:TLR4signaling pathway is involved in the process of liver injury in rats with obstructive jaundice, medium-intensity aerobic exercise in combination with BMSCs or MBD may reduce the liver injury by inhibiting of TLR4signaling pathway and adjusting the expression of liver inflammatory cytokines.