Functionalized Porous Silicon For The Adsorption And Seperation Of Biomolecules

Mesoporous silica has been used in catalysis,adsorption and separation, controlled drug release and biological sensors,etc.due to their higher surface area and large pore volume,affluent morphology,adjustable pore size,easy modification surface and other characteristics.The design and synthesis of different types of mesoporous silica materials and their performance research has been the focus of the field of porous materials research.However,it is difficult for large molecules to disperse and transport in their pores due to the small pore size of mesoporous silica.In the fields involved in biological macromolecules of adsorption,separation,identification and detection,the mesoporous silica are still limited.Although researchers have used various methods to expand the pore size of the mesopores,such as adding reaming agents and using ion etching to expand the pores,the operation is very complicated and expensive.In this paper,meso-/macroporous silica materials are applied to the adsorption,separation and detection of biomolecules,to explore the feasibility of the materials in adsorption and separation of biomoleculars.In this paper,the hierarchically meso-/macroporous silica materials were prepared by cotemplate method,its application in adsorption,separation of biomolecules and influencing factors of adsorption were studied;to explore using CTAB as single template synthesis of hierarchical meso-/macroporous silica nanoparticles,the surface of materials were functionalized with amino,to improve the adsorption and separation properties of biological molecules of the materials;then using molecular imprinting polymer coated magnetic porous silicon material;to carry biological molecular imprinting,studies its application in biological molecular recognition,adsorption and separation etc.;in addition,in order to investigate the application in biosensor technology after adsorption biomolecular,mesoporous carbon materials were designed and prepared by one-step method,and studied biomolecular adsorption properties.The main contents of this paper are summarized as follows:Firstly,hierarchically porous silica materials were prepared by cotemplate method.The hierarchically porous silica was prepared by using the block copolymer P123 as the template and Scylla shell as macroporous template.Scanning electron microscopy?SEM?,powder X ray diffraction?XRD?,high resolution transmission electron microscopy?TEM?and nitrogen absorption desorption were used to characterize the prepared materials.Bovine serum albumin?BSA?and Lysozyme?Lyz?were used as a model biomoleculars,the adsorption propperties of the materials were studied in details.The results show that the existence of material multilevel porous greatly improves the performance of materials adsorbing biomolecules.Materials have high adsorption capacity for two kinds of biomolecules,and show higher adsorption concentration and faster adsorption equilibrium time.A series of hierarchically with ammonia functionalization meso-/macroporous silica nanoparticles were prepared by using the single surfactant CTAB as a template,acetone and water as solvent,3-aminopropyltriethoxysilane?APTES?as ammonia functionalization reagent,tetraethoxysilane?TEOS?as silicon species and by adjusting the amount of APTES,the mechanism of preparation of hierarchically porous silica nanoparticles using a single template are discussed and studied.Finally,BSA and Lyz were used as model biological molecules to study the influence of functional modification of ammonia on the adsorption of biomolecules.The adsorption of BSA and Lyz of the materials shows that the material has high ability to adsorb biomolecules,and the obtained materials can be applied to adsorption and enrichment of biomolecules.Macroporous silicon was prepared by using the crab shell as the template,TEOS as silicon source,and then using ferric nitrate as iron source,macroporous silica magnetic Fe₃O₄ nanoparticles composite was prepared,finally The imprinted and nonimprinted polydopamine coated magnetic porous silicon composite functional material were prepared by using dopamine as the polymer monomer,bovine hemoglobin?BHb?as molecular.The rebinding ability,separation and identification ability,stability and cycling performance of the as-synthesized materials for BHb were systematically studied.The results show that the material has excellent identification and separation performance for the template protein,and the material has a good recycling performance.By using silicon assisted strategy,two different morphologies of mesoporous carbon nanoparticles were synthesized by using CTAB as the template,TEOS as silicon source,phenolic resin as carbon source,the adsorption properties of materials for small biological molecules lysozyme was tested.The results showed that the two kinds of carbon materials showed higher properties of biomolecules,and could be further applied to the preparation of biosensors.