| Lactobacillus is one of probiotic bacteria, and polyphenols is a natural beneficial substances extracted from tea. They have the of functions. This study investigated the long-term effects of oral tea polyphenols (TP) and Lactobacillus brevis M8 (LB) on biochemical parameters, digestive enzymes, and cytokines expression in broilers.480 AA broilers of 1 day old were randomly divided into eight groups, seven experimental groups received different dosage of lactic acid bacteria and tea polyphenols, one control group was fed by gavage with sterile water in whole test period of 84 days.Results:1) Lactobacillus, tea polyphenols, and both of them reduced final body weight and average daily gain (P<0.05). Different dosage of Lactobacillus failed to affect final body weight and average daily gain (P<0.05). While different dosage of tea polyphenols significantly inhibited final body weight and average daily weight gain (P<0.05), and significantly improve the ratio of feed to meat (P<0.05). The results demonstrated that TP and LB failed to improve the growth performance in birds;2) Fed with 1.0ml/(kg.bw) lactobacillus decreased LDL-C, TG, CHO concentration of broilers compared with the control group. Fed with 0.06 g/(kg.bw) tea polyphenols there was significant defference of broilers’LDL-C, GLU, TG, CHO, HDL-C concentration compared with the control group. Fed with both of lactobacillus and tea polyphenols broilers’ LDL-C, TG and CHO concentration were reduced significantly. Low, medium and high doses of lactobacillus combine with polyphenols fed with broilers reduced broiler TG concentration significantly and improved GLU concentration; fed with the group of low, medium and high doses of polyphenols combine with lactobacillus reduced the concentration of LDL-C and TG. The results showed that TP and LB affected serum biochemical parameters, including serum cholesterol (CHO) and low density lipoprotein cholesterol (HDL-C). The results revealed that TP and LB improved serum biochemical indexes and the two has a synergistic effect;3) Broilers fed a diet supplemented with 1.0 ml/kg.BW LB had a lower intestinal lipase activity at day 84 compared with the control group (P<0.05), and were improved of pancreas lipase and gizzard stomach pepsin activity. Fed with 0.06 g/kg.BW TP broilers’ intestinal lipase and glandular stomach pepsin activity were reduced (P<0.05), and pancreas lipase and pancreas trypsin activity were enhanced (P< 0.05). Different dosage of LB supplementation inhibited intestinal lipase activity (P<0.05). Different dosage of TP supplementation improved gizzard stomach pepsin activity (P<0.05). The results revealed that LB and TP improved intestinal digestive enzymes and the two has a synergistic effect.4) The results showed that co-administration of lactic acid bacteria and tea polyphenols significantly increased the growth performance and serum and intestinal IgG and IgM levels (P<0.05). Meanwhile, lactic acid bacteria and tea polyphenols treatment significantly decreased intestinal IL-1β\IL-6, TNF-α, and NF-κB abundances (P<0.05). Compared with LLB group, IL-1β and TNF-α expression were significantly lower in HLB group (P<0.05). Compared with LTP group, IL-1β and NF-κB expression were significantly lower in HLB group (P<0.05). The results revealed that TP and LB improved immune function and inflammatory response and the two has a synergistic effect.5) Supplementation with 1.0 [mL/(kg.bw)] lactic acid bacteria+0.03 [g/(kg.bw)] tea polyphenols had the maximum richness value of bacterial community. In addition, microflora in the broilers received 1.0 [mL/(kg.bw)] lactic acid bacteria and 1.0 [mL/(kg.bw)] lactic acid bacteria +0.06 [g/(kg.bw)] tea polyphenols had been classified into one category and the similar ration is 80%. In conclusion, intragastric administration of lactic acid bacteria and tea polyphenols exerted a potential effect on intestinal microflora in broilers. |
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