Effect And Mechanism Of Epigallocatechin Gallate In Bladder Dysfunction Induced By Bladder Outlet Obstruction

Purpose: To investigate the protective effect of epigallocatechin gallate(EGCG),a green tea extract,and its underlying mechanism on bladder dysfunction in a rat model of bladder outlet obstruction(BOO).Materials and Methods: Sprague-Dawley rats of BOO were surgically induced and followed by treatment with EGCG(5mg/kg/day)or saline(Control)via intraperitoneal injection.Cystometry was performed on four weeks postoperatively in conscious rats.H.E,Masson trichrome and TUNEL staining were performed to observe tissue alterations.Oxidative stress markers were measured and protein expression of Nrf2-ARE pathway was examined by immunohistochemistry and western blotting.Results: Our data showed that EGCG could increase the peak voiding pressure,bladder compliance and prolong micturition interval of BOO rats compared with control and finally reduce the frequency of urinary.EGCG could ameliorate the increase of collagen fibers,MDA and ROS induced by obstruction and increase the activity of SOD,GSH-Px and CAT.The level of cell apoptosis was decreased in BOO rats treated with EGCG compared with control and Caspase-3 expression was reduced as well.Moreover,EGCG could activate the Nrf2 expression with elevation of its target antioxidant proteins.Conclusions: EGCG alleviate BOO-induced bladder dysfunction via suppression of oxidative stress and activation of the protein expression of Nrf2-ARE pathway.