The Expression And Function Of Phosphodiesterase Type 5 And Related Proteins In Bladder Outlet Obstruction Model

BACKGROUND:Lower urinary tract symptoms(LUTS)are one of the common symptoms of older men.LUTS is closely related to bladder outlet obstruction(BOO).Studies have shown that phosphodiesterase type 5(PDE5)is present in the prostate and bladder smooth muscle cells and affects its contractile properties.OBJECTIVE:To investigate the expression of PDE5 and related pathway proteins eNOS and nNOS in bladder smooth muscle cells by BOO rat model and bladder tumor of BOO patients.Methods:The expression of PDE5,eNOS and nNOS were detected by real-time fluorescence quantitative PCR.The expression of PDE5,eNOS and nNOS protein was detected by Western-bolot.The systolic and diastolic characteristics of PDE5 were detected by organ bath.RESULTS:Compared with normal rat bladder smooth muscle cells,PDE5 was highly expressed in the bladder smooth muscle cells of BOO rats.Compared with normal human bladder smooth muscle cells,PDE5 was significantly higher in bladder transitional cell.In both the gene or protein levels are also high expression.2 weeks after obstruction BOO rats bladder smooth muscle hyperplasia,significantly increased contractility;and BOO patients with bladder smooth muscle cells decreased fibrous nodule tissue proliferation,decreased contractility.No matter the bladder tissue of human or rat,the relaxation effect of sodium nitroprusside on the obstruction of bladder smooth muscle tissue is greater than the normal bladder tissue.Conclusion:PDE5 mainly exists in the bladder smooth muscle cell tubal,epithelial cells in the content is very small.The expression of PDE5 in smooth muscle cells after bladder outlet obstruction was significantly increased.PDE5 inhibitors can relax bladder detrusor.