Experimental Study On The Effects Of Activating Blood And Tonifying The Kidney On Inflammatory Cytokines And Adhesion-related Indices In The Rats With Chronic Pelvic Inflammatory Disease

Objective:To explore the immunoregulation mechanism of the treatment of promoting blood circulation and tonifying the kidney on the rats with chronic pelvic inflammatory disease(CPID) by observing the changes of inflammatory cytokines and adhesion related molecules and neuclear factor expression in serum or uterine tissue of CPID model rats.Method:Female Wistar rats were used and the CPID model was established with mixed bacteria injection combined with mechanic injury. Sixty-five rats were randomly divided into normal group, sham operation group, model group, Penqiangyan grannule high, medium and low dose groups and Penyanjing grannule group. The histomorphological changes of the rats'uteri were observed using light microscope. The enzyme-linked immunosorbent assay (ELISA) was applied to measure the concentration of pro-inflammatory cytokines (IL-1?, IL-8, MCP-1) and anti-inflammatory cytokines (IL-10) and transforming growth factor-?1(TGF-?1) in serum of rats in each group; the immunohistochemistry method was used to detect the expression of the following indices in rats'uterine tissue:tumor necrosis factor-?(TNF-?), intercellular adhesion molecule-1(ICAM-1), neuclear factor-kappaB(NF-?B) and inhibitor of nuclear factor-kappaB(I?B). The expression of TNF-?mRNA and ICAM-1mRNA were measured using RT-PCR.Results:The serous levels of pro-inflammatory cytokines (IL-1?, IL-8, MCP-1) and TGF-?1 level in model group were significantly higher than those in normal group (P<0.01); the serous levels of anti-inflammatory cytokines ((IL-4, IL-10) in model group were significantly lower than those in normal group. The expression of TNF-?, ICAM-1, NF-?B and I?B and the expression of TNF-?mRNA and ICAM-1mRNA were all significantly increased than those in normal group. After treatment, the pro-inflammatory cytokine levels were decreased while the anti-inflammatory cytokine levels were elevated. The expression of adhesion related immune molecules(TNF-?, ICAM-1) and NF-?B, I?B in the uterine tissue of model group was significantly decreased (P<0.01). Except for ICAM-1mRNA, all the other indices in Penqiangyan medium and large dose groups were similar to the levels of normal group (P>0.05).In Penqiangyan medium dose group, the expression of NF-?B, TNF-amRNA and TNF-a were correlated significantly, so were the expression of NF-?B, ICAM-1mRNA and ICAM-1.Conclusions:The method of promoting blood circulation and tonifying the kidney could adjust levels of the inflammatory cytokines and various adhesion-related molecules, thus restore the immune balance effectively so as to inhibit inflammation, prevent and alleviate the pelvic adhesions. The mechanism might be that the method could suppress the activation of NF-?B, therefore inhibit the transcription of certain inflammatory cytokines and immune-related adhesion molecule.