The Clinic Effect Evaluation Of Activating Blood And Toniyfing The Kidney On Comprehensive Treating Chronic Pelvic Inflammatory Disease And Expeirmental Study On The Effects On Antiadhesion Of The Experimental Model Rats

Objective:By clinical study, clinical effect on activating blood and tonifying the kidney on Comprehensive Treating Chronic Pelvic Inflammatory Disease was evaluated. Scientific basis was provided for clinical application.By animal experiment,to explore the mechanism of the treatment of promoting blood circulation and tonifying the kidney comprehensive treatment on the rats with chronic pelvic inflammatory disease (CPID) by observing the changes of antiadhesion,experimental basis for traditional medicine synthetically therapy of CPID was provided.Methods:1. Clinical Research:The subject used randomized, multi-center blinded evaluation of test method,139cases of chronic pelvic inflammatory disease of blood stagnate and deficiency of the kidney were divided into two groups randomly; treatment group had67cases that is PEN QIANG YAN Recipe+PEN YAN XIAO enema+dreg sextem alapplication therapy; control group had72cases that is PEN QIANG YAN Recipe. Observe clinical efficiency, Traditional Chinese medicine symptoms physical signs after the treatment.2. Animal Experiment:Female Wistar rats were used and the CPID model was established with sleeping deprived+ring terrified+mixed bacteria injection+mechanic injury. Rats were randomly divided into normal group, sham operation group, model group, taking herbs orally large, medium and small dose groups and comprehensive treatment large, medium and small dose group. The histomorphological changes of the rats’ uteri were observed using light microscope.The immunochemistry method was used to detect the expression of Insulin-like growth factor-1(IGF-1)、Fibroblas t growth factor-2(FGF-2)and Matrix metalloproteinases-2(MMP-2) in rats’uterine tissue.Results:1.Clinical Research:Clinical observation result indicates that the recovery rate of the comprehensive healing effect、traditional Chinese medicine symptom and local sign on activating blood and tonifying the kidney on Comprehensive Treating CPID treatmen had been promoted:45.59%to81.25%,50.00%to81.25%,44.11%to68.75%,two groups relatively have a significant difference (P<0.01). The Clinic Effect Evaluation of Activating Blood and Tonifying the Kidney on Comprehensive Treating Chronic Pelvic Inflammatory Disease is evident effect.2.Animal Experiment:The uterine tissure expression of IGF-1、FGF-2、MMP-2were all significantly increased than those in normal group (P<0.05).After treatment, excepted taking herbs orally small dose groups, The uterine tissure expression of MMP-2in other treatment group were significantly increased compared with model groups (P<0.05or P<0.01); the uterine tissure expression of IGF-1、FGF-2in all treatment groups were significantly reduced compared with model group (P<0.05). Comprehensive treatment large, medium dose group significantly outweighed the other treatment groups on IGF-1、 FGF-2、MMP-2(P<0.01)Conclusions:The ComPrehensive Therapy method of promoting blood circulation and tonifying the kidney is safe and efficient, and suitable for clinical application. The method can adjust the levels of antiadhesion, thus can improve small blood circle,increase effective blood quantity in the pelvic cavity,and remove blood stasis, effectively so as to inhibit inflammation, prevent and alleviate the pelvic adhesions.