Experimental Study On The Effects Of Activating Blood And Tonifying The Kidney On Inflammatory Cytokines Antioxygen And Control Of Cell Apoptosis In The Rats With Chronic Pelvic Inflammatory Disease

Objective : To explore the immunoregulation mechanism of the treatment of promoting blood circulation and tonifying the kidney on the rats with chronic pelvic inflammatory disease(CPID) by observing the changes of inflammatory cytokines, antioxygen and control of cell apoptosis.Methods :Female Wistar rats were used and the CPID model was established with mixed bacteria injection combined with mechanic injury. Fifty-six rats were randomly divided into normal group, sham operation group, model group, Penqiangyan granule large , medium and small dose groups and Penyanjing granule group. The histomorphological changes of the rats’ uteri were observed using light microscope. The enzyme-linked immunosorbent assay (ELISA) was applied to measure the concentration of pro-inflammatory cytokines (IL-6) and anti-inflammatory cytokines (IL-4)in serum of rats in each group; The method of xanthinoxidase were applied to measure concentration of SOD.The method of thio-malonylurea were applied to measure the concentration of MDA.The immunochemistry method was used to detect the expression of Caspase-3 in rats’uterine tissue.The expression of Fas/FasLmRNA and p53mRNA were measured by using RT-PCRResults:The serous levels of pro-inflammatory cytokines (IL-6) in model group were significantly higher than those in normal group(P<0.01); the serous levels of anti-inflammatory cytokines(IL-4) in model group were significantly lower than those in normal group. The serous levels of MDA in model group were significantly higher than those in normal group(P<0.01);the serous levels of SOD in model group were significantly lower than those in normal group(P<0.01).The uterine tissure expression of Caspase-3, Fas/FasLmRNA and p53mRNA were all significantly increased than those in normal group.After treatment ,the serous levels of pro-inflammatory cytokine(IL-6)in all treatment group were significantly lower than those in model group,meanwhile the anti-inflammatory cytokin(eIL-4)in all treatment group were elevated .The serous levels of MDA in all treatment groupwere significantly decreased than those in model group(P<0.01);the serous levels of SOD in all treatment group were significantly increased than those in model group(P<0.01).The coefficient correlation of the serous levels of SOD and MDA in all Penqiangyan granule groups was 0.798,showing the significant negative correlation(P<0.01). The uterine tissure expression of Caspase-3, Fas/FasLmRNA as well as p53mRNA in all treatment group were significantly increased compared with model group(P<0.01). IL-6、IL-4、SOD and MDA in Penqiangyan granule large dose group were similar to the levels of normal group(P>0.05).Except for IL-6 and Caspase-3 Penqiangyan granule large dose group significantly outweighed the Penyanjing granule group(P<0.01). Except for IL-4 Penqiangyan granule large、medium dose groups significantly outweighed the Penqiangyan granule small dose group(P<0.05).The coefficient correlation of the uterine tissure expression of FasmRNA and FasLmRNA in all Penqiangyan granule groups was 0.894,showing the significant positive correlation(P<0.01).Conclusions: The method of promoting blood circulation and tonifying the kidney could adjust the levels of the inflammatory cytokines and the corresponding antioxygen indices,thus restore the immune balance effectively so as to inhibit inflammation,speeding up eliminating free radicles,decrease the lipid,so as to relieve the chronic injury of pelvic tissures and organs.The method of promoting blood circulation and tonifying the kidney could strengthen and induce the expression of cell apoptosis,inhibit the growth of inflammatory corpuscles,thus ease the inflammatory reaction.