Researches On Curculigo Orchioides Gaertn. With The Toxicodynamics And The Toxicities Of Target Organs

Objective:Research the acute toxicity testing,long term toxicity and the action with target organ mechanism of Curculigo orchioides Gaertn. Methods:?Research the performances and LD50 of the acute toxicity with Water and ethanol extractinged from Curculigo orchioides Gaertn.?Set the high dose (120g/kg)?medial dose(60g/kg)?low dose(30g/kg)and the control group, intragastric administration daily dosing 1 times, continuous dosing 30 days?90 days and withdrawal restorative observation 15 days. Observe the general symptoms, blood cytology and blood biochemistry, naked eye, light and electron microscopic observation pathology.?Continue dosing 30 days with ethanol extraction from Curculigo orchioides Gaertn., determin LDH?MDH?SDH?GST?MAO?GDH?IDH?LAP?MDA?-SH?GSH?SOD?FSH?LH and T of the blood serum; Observe the liver?kidney?testis and ovary under the electron microscopic; Using in vitro liver cell culture technology, research the effection of Curculigoside and ethanol extraction from Curculigo orchioides Gaertn.,determin LDH?AST and ALB in hepatocyte supernatant;determin CYP450?b5?CYP1A1?CYP2E1?CYP3A4 in liver microsomal. Results:?Water extraction is safe with clinical daily recommended dosage; Alcohol extraction appearance as less dynamic,the LD50 is 215.9g/kg, liver and kidney performance as hyperemia and edema under the light microscope.?Intragastric administration dosing after 30 days, the AST?BUN?liver and kidney coefficients of high, medial and low dose are higher than the control group (p<0.05-0.01), the ALT and testis coefficients of high dose are higher than the control group (p<0.05-0.01);90 days, the ALT of high, medial dose are higher than the control group (p<0.05-0.01), the BUN and testis coefficients of of high dose are higher than the control group (p<0.05), the CREA and liver and kidney coefficients of high, medial and low dose are higher than the control group (p<0.01), the uterus coefficient of medial dose is lower than the control group (p<0.05); Withdrawal 15 days,there are no significant deviations (p>0.05).Under the light microscope,major organs have no changes.?The LAP?FSH of high and low dose are higher than control group (p<0.05-0.01); b5 and CYP1A1 in liver microsomal are lower than control group (p<0.05-0.01); At the different concentrations and periods, Curculigoside and ethanol extractions from Curculigo orchioides Gaertn. can heighten LDH and ALB in in hepatocyte supernatant;SOD?SH?GSH are lower than control group (p<0.01); Under the electron microscope,the livers and kidneys of high dose have no changes,but the testis and the ovarian present the pathological changes as mitochondria swelling. drug withdrawal 15 days after, each index has no notable difference.Conclusions:?two extractions of Curculigo orchioides Gaertn. in clinical recommended doses are security, ethanol extractions with large doses acutely can cause animals die, liver?kidney and other important organs performanced as hyperemia and edema.?Intragastric administration witrh Ethanol extraction of Curculigo orchioides Gaertn.with high dose and long term, may cause the pathological changes of testis and the ovarian as mitochondria swelling,and it can also change the liver and kidney functions.?ethanol extractions may heighten the LAP and FSH, degrade the SOD?-SH and GSH in blood serum, degrade b5 and CYP1A1 in liver microsomal, heighten the LDH and ALB in in hepatocyte supernatant, cause the mitochondria of testis and ovary swelling and dropsy.