| Objective:Atrial Fibrillation(AF)is the most common and serious arrhythmia in clinical practice,with the incidence increasing year by year.This paper intends to explore and analyze the correlation between the polymorphisms of SCN5A,CETP,Cx40,AGT and eNOS genes and non-valvular atrial fibrillation patients of Uygur nationality in Xin jiang,so as to provide a basis for clarifying the molecular biological pathogenesis of atrial fibrillation.Methods:A case-control study was been used in this article,100 cases of Xinjiang Uygur patients with non-valvular atrial fibrillation(experimental group)and 100 cases of non-atrial fibrillation patients(control group)admitted to the department of general cardiology,the first affiliated hospital of Xinjiang Medical University from February 2013 to August 2015 were selected.Peripheral venous blood was used to extract DNA.Using PCR technology to H558R SCN5A gene polymorphism loci,the CETP gene TaqIB polymorphism loci,Cx40 gene polymorphism loci(-44 + 71 G/A and A/G),AGT gene polymorphism loci,M235T and G-6 A,T174M,eNOS gene polymorphism loci T786C and G894T polymorphism detection,gene polymorphism distribution difference between the experimental group and control group comparison,analysis of genotypes and the correlation between the onset of atrial fibrillation.Results:The age of Uygur non-valvular atrial fibrillation patients was statistically significant compared with the control group.There were statistically significant differences in total cholesterol,LDL-C and uricacid.Compared with the control group,the size of left and right atria was statistically significant,but there were no statistically significant differences in left ventricular ejection fraction and left ventricular end diastolic size.SCN5A gene: the gene sequencing SCN5A gene was mainly AA genotype in the H558R locus,and the distribution of genotypes and alleles in the AF group was significantly different from that in the non-atrial fibrillation group(P<0.05).Logistic regression analysis showed that the G allele might be a vulnerable gene for AF.CETP gene: the differences of genotype and allele distribution between the two groups of CETP Taq IB locus were statistically significant.Cx40gene: Cx40gene--44G/A and +71A/G polymorphism were associated with the occurrence of AF in Uygur patients.Pairwise linkage disequilibrium analysis indicated that the two polymorphic sites were in perfect linkage disequilibrium.The frequency of-44 AA genotype and A allele in AF group was higher than that in control group.Genotyping analysis of the gene model showed that carriers of A gene in the dominant Cx40-44G/A model(GG vs GA+AA)increased the risk of AF.Multivariate logistic regression analysis showed that Cx40 genetic polymorphisms increased the risk of AF in Uygur.AGT gene: the difference of AGT M235T genotype frequency and allele frequency between the Uygur AF group and the control group was statistically significant(P <0.05).The occurrence of AF in Uygur in Xinjiang was associated with AGT G-6A,AGT M235T and AGTT174M loci.eNOS gene: the difference of eNOS G-894T genotype distribution between AF group and control group was statistically significant.The T allele frequency was significantly different between the AF group and the control group.Genetic polymorphism of this gene may increase the genetic susceptibility of Uygur AF.The TT,TC and CC genotype frequencies of T-786C gene in the AF group were not significantly different from those in the control group.Conclusion:The occurrence of atrial fibrillation in Xinjiang Uygur was correlated with gene polymorphisms of SCN5A genes H558R,CETPTaq IB,Cx40 genes-44G/A,+71A/G,AGTG-6A,AGTM235 T,AGTT174M and eNOSG-894T.The G allele of SCN5 A gene is a potential risk factor for non-valvular atrial fibrillation in Uygur patients.There was no correlation between eNOST-786C gene polymorphism and the occurrence of non-valvular atrial fibrillation in Uygur in Xinjiang. |
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