| Functional constipation(F C)is one of the more frequent Functional gastrointestinal diseases in clinical practice,which is a kind of chronic constipation.The clinical manifestations of patients include difficulty in defecation,inexhaustive feeling of defecation,reduced frequency of defecation,dry and hard stool and other symptoms.The exact etiology of F C has not been clear yet,but some studies have suggestted that F C is relatted to the disturbance of intestinal flora.Traditional Chinese medicine has a good effect on constipation.Literature shows that the intestinal flora of patients can be improved by using traditional Chinese medicine to treat constipation.On the one hand,this paper observed the efficacy of self-formulatted Yiqi Yangxue prescription in the treatment of functional constipation through clinical studies,and clarified the regulation effect of this prescription on intestinal flora.On the other hand,animal experiments were carried out to establish a rat model of F C with deficiency of qi and blood,and the self-formulatted formula of enriching qi and nourisshing blood was used to intervene the rat model of F C with deficiency of qi and blood,so as to clarify the regulation effect of this formula on intestinal flora and explore the correlation between constipation and intestinal flora disorders.Clinical Study on the Treatment of Constipation with Deficiency of Qi and Blood Treatted by Yiqi Yangxue Decoction.Objective:To observe and verify the clinical efficacy of self-formulatted Yiqiyangxue recipe in the treatment of functional constipation.Self-formulatted Yiqiyangxue recipe can regulate and improve the intestinal flora of F C patients with deficiency of both qi and blood.Methods:Sixty-two patients diagnosed as functional constipation with deficiency of qi and blood were selectted from the outpatient department of the anorectal department of Dongfang Hospital,Beijing University of Traditional Chinese Medicine.They were drive into treatment group and control group with 31 cases in each group according to random number table method.The treatment group was treatted with self-formulatted Yiqiyangxue prescription orally,and the control group was treatted with multivurate sodium tablet orally.The scores of constipation and TCM syndrome before and after treatment in the two groups were countted.The species composition,diversity and relative abundance of intestinal flora in the feces of two groups of subjects before and after treatment were compared by 16S diversity test,OTU cluster analysis,Alpha and Beta diversity analysis.Results:There were 29 cases in the treatment group and the 28 cases in the control group.There were no adverse reactions in the experimental group and 2 cases in the control group.STThe total effective rate of constipation in treatment group was 89.65%,while that in the control group was 78.57%,and there was statistical difference between the two groups(P<0.05)Spoony parents have been much,filial children and grandchildren who have seen.After treatment,the total score of constipation and the total score of TCM symptoms in the treatment group were lower than before treatment,and the differences were statistically significant(P>0.05);The total score of constipation in the control group was lower than that before treatment,and the difference was statistically significant(P>0.05),there was no statistical significance between the total symptom score of TCM and the ratio before treatment.The intestinal flora of the two groups of subjects was improved after treatment compared with that before treatment,and the diversity was increased.The relative abundance of Mycophyla increased,but the relative abundance of Mycophyla decreased,and the difference was statistically significant(P>0.05);On the genus level,The relative abundance of genus increased,while that of phyla decreased,and the difference was statistically significant(P>0.05),and the improvement of the treatment group was better than that of the control group(P>0.05).Conclusion:Self-formulatted Yiqi Yangxue Decoction has definite clinical efficacy in the treatment of F C with deficiency of qi and blood,and can restore the intestinal flora of patients.Experimental studyOBJECTIVE:To establish the model of F C rats with deficiency of both qi and blood,and verify the regulating effect of self-formulated formula of enriching qi and nourisshing blood on intestinal microflora of F C rats with deficiency of both qi and blood.METHODS:50 rats were divided into normal group,model group,control group,Chinese medicine low-dose group and Chinese medicine high-dose group with 10 rats each.To 4 groups after two qi type F C rats model was constructed,the judge made after the success of the module,the control group using multiple libraries ester sodium therapy,Chinese medicine high dose and low dose group using the quasi tomifying qi and blood treatment,the normal group and model group rats with only normal diet,the rats were observed before and after the intervention group generally,animal feces,intestinal flora.The species composition,diversity and relative abundance of intestinal flora in feces of rats in each group before and after intervention were compared by 16 diversity tests,OTU cluster analysis,Alpha and Beta diversity analysis.RESULTS:After modeling,the general condition and feces of rats in model group,control group,low-dose group and high-dose group were significantly different from those in normal group(P>0.05),there were no significant differences in general condition,24-hour fecal quantity and weight among the first 4 groups(P<0.05).After modeling,the intestinal microflora diversity of model group,control group,low dose and high dose Chinese medicine group was lower than that of normal group.At the phylum level,the abundance of Bacteroidetes,Proteobacteria,Radiobacteria decreased compared with the normal group,while the abundance of Firmicutes,Microflora verrucosa increased,and the difference was statistically significant(P>0.05);At the genus level,the abundance of Blaautia,Coprococcus,Clostridium and Ruminococcus increased compared with the normal group,while the abundance of Clostridium and Bifidobacterium,which were not classified,decreased,with statistical difference(P>0.05),the abundance of Lactobacillus and heterobacter showed no statistical significance compared with normal group.After drug intervention,the abundance of Firmicutes and Microflora decreased,while the abundance of Bacteroidetes,Proteobacteria and Radiobacteria increased.At the genus level,after intervention,the abundance of Blautia,Clostridium,Clostridium unclassified,Coprococcus and Ruminococcus decreased in the two groups treated with traditional Chinese medicine,while the abundance of Bifidobacterium increased compared with before intervention,while the abundance of Lactobacillus and heterobacterium showed no statistical significance.In the control group,the abundance of Clostridium,Clostridium unclassified and Coprococcus decreased,while the abundance of Bifidobacterium increased,while the abundance of Lactobacillus,Bacteria,Ruminococcus and Isobacter showed no statistical significance.The intestinal flora of the two groups treated with traditional Chinese medicine showed no statistical significance after intervention(P<0.05).CONCLUSIONS:After modeling,intestinal microflora structure of FC rats with deficiency of both qi and blood is disorder,and bacterial diversity is decreased.Self-formulated Yiqi Yangxue Decoction can improve bacterial diversity,restore intestinal microflora structure,increase the abundance of beneficial bacteria,and reduce the abundance of harmful bacteria.The improvement of intestinal microflora is better than that of Dokurate sodium tablet. |
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