Nourishing Huoxue Recipe Clinical Research Of The Treatment Of Blood Deficiency Constipation And Constipation Mouse Model Of Colon Icc

Doctors and patients often ignore the disease of chronic constipation because its harm is gradual.Even that we shouldn't disregard of the jeopardise caused by chronic constipation.Many of patients think that the constipation is only difficult to defecate,in fact chronic constipation can result in so many kinds of diseases.With the change of drink and food,effect on mind and society,chronic constipation severely impact on quanlity of life in modern society.Chronic constipation is tightly relative to colon carcinoma,hepatocerebral disease(HCD),breast disease,presenile dementia and so on,Chronic constipation can result in emergency of life if someone got sick of acute myocardial infarction or cerebral accident.Some of patients with chronic constipation are prone to hemorrhoids and anal fissure.Accordingly,earlier prevention and rational therapy will greatly cut down the severe consequence and society burden resulted by constipation.With the elevation of life level,people hope to live more comfortable life and think highly of treatment on functional disease.Incidence of constipation gradually goes up,especially among the aged people and multiparous women because of the transform of life style and the change of diet composition.Data of epidemiology means that incidence of chronic constipation is 2%in USA and increases by age.The data told us that there are 4 millions people to get sick of chronic constipation every year and 2--3 millions patients with chronic constipation to defecate by luxative.Data of epidemiology in China is more than 2%,even that is different from city to city.There are so many kinds of drugs to treat on chronic constipation,but its therapeutic effect is difficult to last for long time and long-term application of medicine will result in drug addiction and side effect.Experts of Traditional Chinese medicine(TCM) accumulate a lot of experiences in treatment on chronic constipation and devided it into 2 types of deficiency and excess through differentiation of symptoms and signs.Most of patients with refractory constipation are the blood deficiency,especially among older or multiparous women patients through differentiation of symptoms and signs.Professor Li who is a famous expert for diseases of colon and rectum discovered that main reason of blood deficiency type refractory constipation is stagnation of blood.Professor Li conclude that stagnation of blood aggravates its deficiency.Professor Li researched and created herbs of yang xue huo xue fang(YXHXF) based on his experience in treatment on refractory constipation.Clinical observation found that herbs of YXHXF is effective for chronic constipation of blood-deficiency type.In order to study the mechanism of herbs of YXHXF,we designed the research program which include 2 sections.Section one:clinical trialObjective:to observe therapeutic effect of herbs of YXHXF for chronic constipation Method:80 patients with chronic constipation of blood-deficiency type were divided randomizedly 2 groups.There are 50 patients to take medicine of herbs yang xue huo xue fang(YXHXF) in observation group,Control group of 30 patients take medicine of maren soft capsule.2 weeks for course of treatment,we recorded the change of symptom scores.Result:Through clinical trial,we concluded that effect of YXHXF to treat on chronic constipation of blood-deficiency type is precise and surpass control group.Section two:empirical studyExperiment 1:Duplicate mouse model with chronic constipation of blood-deficiency typeObjective:make mouse model with chronic constipation of blood-deficiency type Method:Combination with literature reports and experience of experiment,we apply with hypo-dermic injection of hydrochloric acid morphine 2.5mg/kg/d for 45 days to make mouse model of constipation.While hypo-dermic injection of hydrochloric acid morphine,we cut off tails of mouse to loss blood for 7 times(one time for every 6 days) to make model of deficiency of blood.Result:successful mouse model of blood-deficiency type constipation showed that tool-weight decreased obviously.Mouse stool turned so sticking and stagnant that it's difficult to fall from anal.In brief,symptoms of mouse model of constipation is consistent with clinical patients with constipation.Experiment 2:the influence of YXHXF on interstitial cells of Cajal(ICC) of mouse model with chronic constipationObjective:to study the mechanism of treatment on chronic constipation of blood-deficiency type by YXHXF.Method:mouse model took medicine of YXHXF,maren soft capsule and mosapride by intragastric administration for 2 weeks and then executed mouse to excise colon specimen.We applied with immunohistochemistry technology to analyze the amount of ICC.Result:The amount of ICC decreased obviously in colon of mouse model with chronic constipation.After treatment by YXHXF,the amount increased more than control group.Experiment 3:the influence of YXHXF on c-kit of colon tissue for mouse model with chronic constipationObjective:to study the mechanism of YXHXF treatment on chronic constipation of blood-deficiency type in molecular levelMethod:we analyzed the diversity of c-kit in mouse colon tissue with chronic constipation by western-blot technology.Result:The density of c-kit decreased obviously in colon of mouse model with chronic constipation.YXHXF can increased the amount of c-kit and the effect is precise,a contrast to control group.