The Effect Of The Oxidoreductase Preparation Treatment On Virginia Tobacco Quality

The oxidoreductase was separated from the fresh top leaves from tobacco agriculture by-production and putted into the Virginia tobacco leaves.The study showed that the rude oxidoreductase separated from the raw material could be purified well by Bio-Gel P-60 column and DEAE-Sepha-dex column. The proportion of after-purified oxidoreductase was 49 percent of rude oxidoreductase,the times of purification reached to 7.6,and the oxidoreductase vigor was 51.8U/mg. The best work condition of oxidoreductase was at 36 and pH6.0.The study also indicated that after treatment by oxidoreductase,the routine chemical components in tobacco changed a lot,which mainly presented on the distinct reducing of contents of sugar,protein,nicotine,chlorophyll and total nitrogen,while the evident rising of organic acid. In addition,some large molecular components such as high fatty acid were decomposed to aroma components,which could be indicated from the result of GC/MS analysis. The result showed some important micro- aroma components proportion raised,such as -damaston,while some components disadvantaged to tobacco smell reduced.The sense evaluation also displayed that the aroma quality and quantity were all improved after treatment with oxidoreductase. In the meantime,the after-taste of Virginia tobacco became clean,the non-tobacco smell was declined and the irritancy was reduced. All these showed the total smoking quality of tobacco was improved,which made the after-treatment tobacco more accectable.