Study On The Extraction And Purification Process Of Glycyrrhiza Polysaccharides From Licorice

The radix and rhizoma of licorice are the most common and important Chinese traditional medicine. Licorice contains the compounds such as triterpenoids, flavonoids and polysaccharides and so on. Glycyrrhiza polysaccharides(GPS) have the extensive biology activity and the great exploitative potential. It can be expected to be the new medicine.Only flavonoids and glycyrrhiza acid was paid attention to be extracted from licorice ago, but the important activity of GPS was neglected . It caused waste because GPS was threw away as residue . Considering it, researches had been done to the extraction of GPS from different materials (extraction extractant of supercritical CO2, residue of extracting flavonoids, residue of extracting flavonoids and glycyrrhiza acid, ultrasonic extractant of residue of extracting flavonoids,radix and rhizoma of licorice) and deeply and systematically researches had been done to the extraction and purification of GPS from the residue of extracting flavonoids. The new process for extraction and purification is decided.The condition of the process as follows:1. Extraction of GPS Use water to dip raw material for 3 hours, the volume of the water was 30ml/g raw material for one time, the temperature 80℃, repeated 3 times. The content of GPS is 40.00% and the yield is 5.40%.2. Purification of GPS The large-diameter adsorbent resin removing pigment, the method of Sevag removing protein, deionized water dialysis removing the impurity of small molecule was used in the purification of GPS and the further purification was finished using the column chromatography of DEAE-32 and Sephadex G-75. The content of GPS is 96.15% and the yield is 0.57%.This process used the licorice resource sufficiently, extracted and purified GPS from residue of supercritical CO2 extracting flavonoids effectively, and provided the strong base of theory and practice to develop polysaccharides medicine.