| In general induction of this thesis, the history, the basic principles, characteristics and the main systems of chemiluminescence (CL) are briefly introduced and the applications of CL systems in pharmaceutical analysis in last ten years are intensively reviewed. In the research section, the new flow injection chemiluminescence(FI-CL) methods for the determination of menadione sodium bisulfite (MSB), dipyridamole(DIP), isoniazid and ascorbic acid have been developed.The goal of this thesis is to develop a series of new flow injection CL methods for the detection of Pharmaceuticals with highly sensitivity. The objective is to explore the pharmaceutical enhanced CL scheme of the follow-up CL reaction of luminol, which we found, and to develop the FI-CL methods for the determination of menadione sodium bisulfite (MSB), dipyridamole, isoniazid and ascorbic acid.CL analysis is becoming increasingly promising method for biomedical, environmental analysis owing to its simple instrumentation, high sensitivity, wide dynamic range, reproducibility, simplicity and rapidity. In recent years, various strategies for improving the selectivity of this method have been continued. Efforts to new CL reactions and this method coupled with the other analytic techniques make it very attractively.In the research section, a scheme of follow-up CL reaction of luminol was proposed. It is believed that the light-emitter is Aminophthalate, which was produced by the first CL reaction of luminol with an oxidant and its exciting energy transferred from the reaction of the oxidant with analyte pharmaceuticals.A novel and simple FI-CL method for the determination of MSB was developed based on the follow-up CL reaction of luminol with KIO4 We found that when menadione sodium bisulfite (MSB) was injected into a CL reaction-finished mixture of luminol and KIO4, a strong CL signal was produced. The analytical conditions including the length of the mixing tube and concentration of all reagents were optimized. Under the optimized condition, the CL signals responded linearly to the concentration of MSB from 2×10-9 g/mL to 4×10-5 g/mL with the detection limit of 8X 10-10 g/mL MSB. The relative standard deviation was less than 0.5% for 1×10-6g/mL MSB(n=ll). The developed method has been applied to determine MSB in the MSB injection samples with satisfactory results. Additionally, a scheme of follow-up CL reaction of luminol, in which lumiol was used as a CL precursor and KIO4 was used as an oxidant and analyte MSB was used as energy-transfer precursor, was proposed.A novel FI-CL method for the determination of dipyridamole(DIP) was developed based on the follow-up CL reaction of luminol with K3Fe(CN)6. When DIP was injected into a CL reaction-finished mixture of luminol and K3Fe(CN)6, a new CL reaction was initiated to produce strong CL emission. The CL intensity responded linearly to the concentration of DIP in the range from 1×10-10 to 4×10-7g/mL with a detection limit of 6×10-11 g/mL. The relative standard deviation was 1.1% for 1×10-8 g/mL DIP solution( n=7). The method has been applied to determine DIP in tablets and the results are in agreement with traditional method using UV spectrometry. Additionally, a scheme of follow-up CL reaction of luminol, in which luminol was used as a CL precursor and K3Fe(CN)6 was used as an oxidant and analyte MSB was used as energy-transfer precursor, was proposed.A simple FI-CL method for the determination of isoniazid was developed based on the follow-up CL reaction of luminol with KMnO4. It was found that a strong CL signal was produced when isoniazid was injected into a CL reaction-finished mixture of luminol and KMnCV Under the optimized condition, the CL signals responded linearly to the concentration of isoniazid from 5×10-8 g/mL to 1×10-4g/mL with a detection limit of 1×10-8g/mL. The relative standard deviation was 1.1% for 1×10-6 g/mL isoniazid (n=7). This method developed has been applied to determine isoniazid in tablets with satisfactory results. By the study of the CL spectrum, Fluo...
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