Studies On Extracting Technology Of Polysaccharide From Jujuba Mill And Preliminary Exploration For Its Function Evaluation

Zizyphus Jujuba Mill belongs to Zizithus Mill of Rhamnaceae .China is the earliest country which cultivates Jujuba tree in the world. Jujuba Mill has a high nutritional value, which contains sugar, cellulose, vitamin, amino acid, protein, minerals, polysaccharide, rutin, cyclic adenylie acid (C-AMP) etc. nutritious components that make Jujuba Mill have all kinds of function.In this paper the technology of extracting polysaccharide from Jujube Mill, precipitation conditions, method to remove protein, and separation with the column of DEAE-cellulose have been studied. And the function of polysaccharide from Jujuba Mill has been evaluated.With the improvement of living standard and rise of health consciousness, the safety, nutrition, hygiene and function properties of food are more and more emphasized. So it is necessary to study the technology of extracting effective ingredient from Jujube Mill and evaluate its function.The test results showed that:1. The optimum conditions of extracting polysaccharide from jujube Mill with the water as following: adding water volume is 15 times as much as that of sample, extracting 6h at 100℃, for two times. The extracting rate was 3.76%.2. The suitable enzyme with extraction by enzymatic technique is cellulose. The optimum conditions are pH 4.5, with 0.02% the enzyme amount, extracting 2h at 60℃, and can acquire polysaccharide 3.91 gram from 100 gram jujube Mill.3. The optimum extracting condition in ultrasonic field is power 12w/cm2, 10 min. The extracting rate was 3.05 %.4. The optimum precipitation conditions ware obtained as follows: The extracting liquid contracted 4 times, adding 4 times volume ethanol and making the final concentration of alcohol 80%.5. The optimum method to remove protein from polysaccharide from Jujube Mill is sevag and must remove protein 7 times.6. Using DEAE-Cellulose to separate the polysaccharides from jujube Mill, five content has been acquired.7. The polysaccharides from jujube Mill can raise the activities of SOD, CAT, and decrease the amount of MDA in blood, liver and brain of rat.8. The polysaccharides from jujube date can restrain the rising of total cholesterol (TG) and triglyceride (TG) in the rat serum, and restrain the declining of serum high density lipoprotein cholesterol (HDL-C).