| Pirns armandi Franch.var.armandi yielding the seed kernel of pinus armandi was densely rooted in our country.but little study on the seed kernel oil of pinus armandi was carried over the time.this research attempts to make a systematic research on the seed kernel oil of pinus armandi and on its function evaluation on the polyunsaturated acids seperated from it, which would pay a way to the exploration and production of the seed kernel oil of pinus armandi.The composition of fatty acids in the seed kernel oil of Pinus armandi was analysed by GC. The oil consisted mainly of linoleic acid 63%, oleic acid 26%, palmitic acid 5%, stearic acid 2%, arehidic acid 1%, erucic acid 1%. The total amount of the unsaturated fatty acid was 90%. Physical and chemical tests results showed that specific gravity is 0.915 and refactive index is 1.4778 and acid value is 5.1 and saponitication value is 190.8 and iodine value is 142.6 and POV isl.217meq/kg.The results showed that the oil contents of the seed kernel of Pinus armandi was 56.5%. After a severe examination of several sorts of extraction solvents, it showed that light petroleum(30-60℃) was optium for the examination, meantime ultrasound wave treatment was a rather fitting approach to extract the seed kernel oil of pinus armandi. The seed kernel of Pinus armandi was treatmented by ultrasound wave for enchancing the extraction rate of pine nut oil. Effect of several factors (ultrasound wave intensity, treatment time, solution ratio, times) on ultrasound wave processing was explored. Obtained the optimum conditions of processing. The results showed that it is under the conditions (ultrasound wave intensity 200KW/m2, treatment time 15 min, solution ratio 1:7 (g:ml),treatment times 2) that extraction rate of pine nut oil was better.Unsaturated fatty acids, especially linoleic acid are rich in the seed kernel oil of Pinus ar/wawft.Uunsaturated fatty acids were separated and purified from the seed kernel oil of Pinus armandi via urea clathration .GC results showed that linoleic acid increased from 63% to 93%, oleic acid decreased from26% to 6% after one time urea clathration. Unsaturated fatty acids amount was up to 99%. Urea clathration was an effective method to separate unsaturated fatty acids from the seed kernel oil of Pinus armandi. The optimum urea clathration conditions were:alcohol serve as solvent, fatty/urea=l:1.5, clathration temperature O'C.Antioxidation research was made in oven (63 ± 1℃). Several antioxidants wasadded to the seed kernel oil of Pimts armandi. The results showed butylated hydroxytolene (BHT), propyl gallate(PG) and tertiary butylhydroquinonoe (TBHQ) were effective antioxidants. TBHQ was more effective than any others. Synergist tests showed that 50mg/kg lemon acid can improve antioxidation of TBHQ. VE had no significant antioxidation.The function evaluation on the unsaturated acids (i.e. consisting of linoleic acid93%, oleic acid 6%, linolenic acid 0.9%, they were separated and purified from the seed kernel oil of Pinus armandi via first urea clathration), was made through pouring stomach for the high fat chow group. The results showed that the level of the serum TG was far lower than that of high fat chow control group (middle dose groupP < 0.05, high dose group P < 0.01) and that the level of the serum TC was even lower than that of high fat chow control group (low dose group P < 0.05, high and middle dose group P < 0.01) and that the level of the serum HDL-C was much lower than that of high fat chow control group (middle dose group P < 0.05, high dose group P < 0.01) and that the level of the serum LDL-C was much lower than that of high fat chow control group (low dose group P < 0.05, high and middle dose group P < 0.01) and that the index of AI was lower than that of high fat chow control group (low dose group P < 0.05, high and middle dose group P < 0.01).It is concluded that the unsaturated acids in seed kernel oil ofpinus armandi could prevent from rats' hyperlipemia and arterioseclerosis.Concerning the memeory capacity of rat, it...
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