The New Coupling Technology Of Lactic Fermentation And Extractive

In this paper, the breeding of lactic acid-resistant stains, culture condition on the immobilized fermentation and coupling technology of lactic fermentation and Extractive was studied.The most lactic acid-resistant stain Lactobacillus delbrueckii LD-1 was selected from two original L.delbrueckii LD-1,LD-2.LD-1-1 could resistant 14g/L lactic acid in the ferment medium through Lactic- resistant domestication. A simple and fast method was built for breeding lactic-resistant stain utilizing lactic acid feedback and resistant to stain. In this method not lactic acid-resistant stain was eliminated through 36 hour cultivate without counteract reagent added and lactic acid-resistant stain was rejuvenated and multiplicated with CaCO3 added after 36 hour.By means of convention batch fermentation, immobilized fermentation was studied. The result showed that carbon was the best material. A maximum yield was obtain under the following immobilized fermentation: temperature 48 C, carbon 20g/L, rice starch saccharification of 140g/L,bran 20g/L.On lactic acid optimum fermentation, the yield of 93.3g/L lactic acid, sucrose conversion of 94% after 72 hour. Under the same condition, free-cell fermentation yields of 93.3g/L after 72 hour. Immobilized fermentation could substitute free-cell fermentation in the coupling technology of lactic acid fermentation and extractive.The characteristic of anion-exchange resin was studied. It showed that the alter-M] anion-exchange resin few were damaged, discolored, absorbed glucose and pigment, etc. At the same time, alter-Mi didn't affect the cell growth. Glucose and cell in the medium did not effected exchangeable amount of Lactic acid. The alter-Mi anion-exchange could be used to coupling technology of lactic fermentation and extractive.The coupling technology of carbon immobilized lactic fermentation and alter-Mi anion-exchange extractive were studied. The optimum conditions were ascertained. The maximum yield was obtain under the following conditions: glucose 140g/L, exacting when the concentration of lactic acid was 6g/L, the velocity of extractive flow is 240ml/h, Under the optimum techniques condition, the new technology reduced time to 60 hour. Product lactic acid was pulled off from alter-M1 anion-exchange and refined. The reclaiming rate is 86%, productivity is 2.01g.L-1.h-1, all physics and chemical character of product up to grade. Chromed is below 1 degree and was evidently better than the now technology.