| This thesis makes up of two primary parts: First, the interaction analysis between single drug and receptor by capillary electrophoresis. Second, the separation and analysis of active ingredients from traditional Chinese medicines (TCM) by capillary electrophoresis. The first part includes chapter 3 and chapter 4, and the second part includes chapter 5 and chapter 6.In chapter 3, β-CD was selected as artificial receptor, and the interaction of citalopram and β-CD was studied using the mobility shift model of capillary electrophoresis interaction analysis. Three different linear plotting methods, namely double reciprocal, y-reciprocal and x-reciprocal were used to estimate the binding constant, 1.620×102,1.425×102 and 1.404×102 L·mol-1 were obtained respectively. This research can give guidance to the further study on the interaction of drugs and their receptors.In chapter 4, human serum albumin (HSA) was selected as general receptor, and the binding constant between sodium houttyfonate (SH) and HSA was determined by capillary electrophoresis. The three same methods as chapter 2 were introduced to calculate the binding constant, 4.072×104,3.845×104 and 3.791×104 L·mol-1 were obtained respectively. These results confirm that the interaction between SH and HSA is strong, and the binding ratio is 1:1, which can provide important information for further studies of the transportation, distribution and activity of SH in human bodies. In chapter 5, a capillary zone electrophoresis (CZE) was developed for the separation of chlorogenic acids from Flos Lonicerae. Analysis was performed on a polyimide-coated fused silica capillary of 48.5 cm effective length,60 cm total length and 50 μm i.d.. The detection wavelength was 324 nm. According to the experimental results, the optimum separation condition was selected as fallows: 10 mmol·L-1 phosphate, 20 mmol·L-1 boric acid, and 10% ethanol (v/v), pH 8.30, as buffer solution, and 25 kV as applied voltage. Under this experimental condition, the extracts were separated satisfactorily. This method could be applied to the ingredient analysis and further quality control of Flos Lonicerae and its medicinal preparations. The interaction between chlorogenic acids and bovine serum albumin (BSA) was also studied roughly, which could give some guidance to the further study on the pharmacodynamics and phamacokinetics of chlorogenic acids in Flos Lonicerae. The work in chapter 6 is to introduce a new micellar electokinetic capillary chromatography method (MECC) for the separation of alkaloids extracted from Kunming Shanhaitang Pian, which is one troche preparation of Tipterygium hypoglaucum (Lévl.) Hutch. The buffer solution employed was 20 mmol·L-1 borate, 30 mmol·L-1 SDS and 30% methanol, pH 8.00. The capillary used was 75 μm in i.d., 39 cm in effective length, 50 cm in total length. UV detection wavelength was 249 nm. The voltage was 20 kV. Under this experimental condition, the extracts were separated to eight active ingredients (or active sites) in 25 min. The developed method could be applied for the ingredient analysis and quality control of Tipterygium hypoglaucum (Lévl.) Hutch and its preparations.Deficient in stern detached quality criteria and reliable scientific data to verify it's effectiveness and security, TCM is still out of the main international medicine market. Combining the two strong functions of CE, that is to say, interaction analysis and ingredients separation, the simultaneous studies of pharmacodynamics and active principles of TCM could be realized, which will help to broaden application range of CE, and to accelerate modernization of traditional Chinese medicines.
|
PDF Full Text Request