| A capillary electrophoresis (CE) with electrochemiluminescence detection (ECL) has potential application in analytical chemistry due to both the high efficiency of separation of capillary electrophoresis and the high sensitivity of ECL detection. The numerous advantages of CE-ECL technique include fast separation, less pollution, decreased cost and waste production.The thesis is concentrated on the development and improvement of CE-ECL separation and detection technique, exploring its extensive application in pharmaceuticals and biochemicals and establishing novel systems of CE-ECL for the determination of drug from metabolized urine and different active ingredients in Chinese traditional medicine, as described below:1. An original method based on capillary electrophoresis- electrochemiluminescence detection was used for the determination of ranitidine. ECL end-column detection of analyte was performed in 5mM Ru(bpy)32+. Detection of ranitidine was confirmed by standard addition method, where the increase of peak height at certain migration time was directly proportional to the amount spiked with drug. The proposed method was applied satisfactorily to the determination of ranitidine in urine.2. The coupling of Ru(bpy)32+ based ECL detection with CE was developed for the simultaneous determination of the two major active ingredients in Flos datura. Validated method shows high sensitivity and resolution for the determination of analyte with detection limits of 5×10-8 mol/l for atropine and 1×10-6 mol/l for scopolamine, respectively. Compared with traditional CE method, the present method displayed good performance in terms of sensitivity. Thus, a special attention is focused on the electropherograms, which can also be used for expressing a characteristic fingerprint for Chinese traditional medicine in the future.3. Capillary electrophoresis / Ru(bpy)32+ electrochemiluminescence (CE-ECL) was established for the determination of four bioactive constituents in Chinese...
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