Study On The Separation And Analysis Of Several Active Components Of Traditional Chinese Medicine By Capillary Electrophoresis

The capillary electrophoresis method has the advantages of rapidity,sensitivity,multiple separation modes,low sample consumption,low cost of separation analysis,etc.In this paper,capillary electrophoresis is used as a research method,established a new method for the separation and analysis of the main active ingredients in four kinds of Chinese herbal medicines and their preparations after detailed optimization of the electrophoretic media and instrument conditions,the details are as follows:1.A capillary zone electrophoresis method was developed for simultaneous separation and determination of forsythin,forsythiaside A and forsythiaside B.The three compounds could be separated and determined in a running buffer?pH 9.42?consisting of 30%?v/v?acetonitrile,20mmol/L Na₂B₄O₇ and 12mmol/LNaOH and in the instrumental conditions of the separation voltage of 22kV,capillary column temperature of 25?,detector wavelength of 280nm,and injection of 5s at 0.5 p.s.i.The method can achieve baseline separation and effective determination of target components within 17minutes.Each component to be tested have a good linear relationship within range of2.5⁸0.0?2.5¹00.0 and 2.5¹00.0?g/mL in the peak area and mass concentration.The method was applied to the determination of three target components in Forsythia nuts,husks,and Lianqiaobaidu Tablet with the recovery ranged between 95.9%¹04.1%and the relative standard deviation of below 5.0%.2.A simple and fast capillary zone electrophoresis method was developed for simultaneous separation and determination of Glycyrrhizic acid,glycyrrhetinic acid,glycyrrhizin,isoliquiritigenin,glycyrrhizin,isoliquiritigenin,licoflavone A,and glycyrrhizin.The eight compounds could be separated and determined in a running buffer?pH 11.34?consisting of 20mmol/L Na₂B₄O₇ and 44mmol/L NaOH and in the instrumental conditions of the separation voltage of 25 kV,capillary column temperature of 25?,detector wavelength of 254nm,and injection of 7s at 0.5 p.s.i.The method can achieve baseline separation and effective determination of target components within 13 minutes.Each component to be tested have a good linear relationship within range of 30.0¹50.0?2.5¹00.0?5.0¹00.0?5.0¹00.0?1.25¹00.0?2.5¹00.0?5.0¹00.0 and 5.0¹00.0?g/mL in the peak area and mass concentration.The method was applied to the determination of eight target components in Licorice and Compound Liquorice Tablet with the recovery ranged between 94.3%¹06.7%and the relative standard deviation of below 5.1%.3.A micellar capillary electrophoresis method was developed for simultaneous separation and determination of madecassoside,asiaticoside,madecassic acid,and asiatic acid.The four compounds could be separated and determined in a running buffer?pH 9.72?consisting of 40%?v/v?methanol,16mmol/L SDS,20mmol/L Na₂B₄O₇ and28mmol/L NaOH and in the instrumental conditions of the separation voltage of 25 kV,capillary column temperature of 25?,detector wavelength of 214nm,and injection of7s at 0.5 p.s.i.The method can achieve baseline separation and effective determination of target components within 41 minutes.Each component to be tested have a good linear relationship within range of 25.0⁴00.0?25.0⁴00.0?25.0⁴00.0 and 25.0⁴00.0?g/mL in the peak area and mass concentration.The method was applied to the determination of three target components in Centella and Sanjin Tablet with the recovery ranged between 96.7%¹03.6%and the relative standard deviation of below5.0%.4.A micellar capillary electrophoresis method was developed for simultaneous separation and determination of psoralin,coryfolin,corylifolinin,psoralidin and corylin.The five compounds could be separated and determined in a running buffer?pH 9.31?consisting of 25%?v/v?acetonitrile,25mmol/L SDS,20mmol/L Na₂B₄O₇ and 4mmol/L NaOH and in the instrumental conditions of the separation voltage of 20kV,capillary column temperature of 25?,detector wavelength of 300nm,and injection of 5s at 0.5p.s.i.The method can achieve baseline separation and effective determination of target components within 23 minutes.Each component to be tested have a good linear relationship within range of 2.5¹25.0?2.5¹25.0?2.5¹25.0 and 2.5¹25.0?g/mL in the peak area and mass concentration.The method was applied to the determination of three target components in Psoralea,Yubiaobushen Pill,and Compound Psoralea Particles with the recovery ranged between 95.4%¹04.7%and the relative standard deviation of below 5.0%.