Studies On Vitamin C Fermentation Process

The text studies systematically the problem about vitamin C industrial ferment technique of The North China Pharmaceutical .,LTD.After studied amount of information about the vitamin C ferment technique. Studied the effect of quality of the one step liquid to the second step fermentation rate and periods first time, confirmed the effect factor of the one step leavens process versus the second step.After orthogonal test we found that no mold air ratio and lodge time mainly affect fermentation of 2- keto - L- gulonic acids among the four agents : sorbital concentration, no mold air ratio lodge time and sterilization temperature . Via antitheses test, The 2- keto - L- gulonic acids content of fermentation liquid in the second step fermentation process increased 1-2mg/ml then primary technics after the no mold air ratio were controlled firmly as 1:0.6 and the lodge time (6h) of the first step fermentation liquid were firmly controlled.Base the quality of the first step fermentation liquid were jarless, the cardinal affecting the agent on fermentation process were systematically studied.1 First the effect of the organic nitrogen source corn plasm of the culture medium were studied ,and the optimization technics assembly were found out, In two different growing period for fermentation tank, the corn plasm add to proportion request difference.Also studied the effect of the corn plasm from different plant.Ascertained the cardinal corn plasm supplier.2 Then the effect of temperatures on the second fermentation process were studied, the actual technical temperatures parameter were optimized.It is advantaged to accumulate 2-keto - L- gulonic acid by adjust the temperature during the growth process.3 After optimization gradient of temeprature parameter , approbate to study, The process of industrial art of large produce was further optimizated by study effects of the dynamic control pH versus each growing period on the second step fermentation process .The research expresses that in different growth period to control the pH value of various cultivating period and to help the accumulation of 2-keto-L-gulonic acid and the shortening of production period..4 After the above agent all the hypothesis of the optimization assembly, versus dynamic variance that different the growing period air ratio was studied, the air proportion affecting organisms used in two-step fermentation process and decide the air proportion for different cultivating period and investigate to what extent air may affect the organisms used in two-step fermentation process so that the fair term was created for 2- keto -L- gulonic acid of the accumulation.In conclusion, If the volume of the first step and second fermentation liquid were measured guarantee errors at 1% including, Aim at the current single canister to proceed the hypothesis that optimization combine control in time next, the tout a fait can be made present I the corporate and single canister produces the accumulation of the 2- keto - L- gulonic acid obtains to leading the level 84～ 86% of the period and can also be controlled at the same time within the sphere of efficient 36～40 h.The antitheses result of examination in 20 m3 fermentation tank is : originally industrial art average conversion rate 81.21%, leaven the period 46.8 hour;Optimization assembly industrial art process average conversion rate 83.81%, leaven the period 38.2 hour, treating average conversion rate than check against to increase 2.6%, leaven the period shortens 8 hour.The further appliance homogeneity design software analyzes, the optimization combined above of the result of research, The yield of the canister inside continuously ten batches leaven increases to 84% above, the jarless leaven period comes 36 ～38 hour at the 100 m3 leavens tank...