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Study On Isolation, Extraction And Biological Activity Of Antioxidant Subtance Of Sweet Potato

Posted on:2005-11-30Degree:MasterType:Thesis
Country:ChinaCandidate:H G HuangFull Text:PDF
GTID:2121360125969502Subject:Botany
Abstract/Summary:PDF Full Text Request
Nutrition value of sweet potato and function of profiting life and resisting tumour were approbated by medical expert and nutritionist . Compared with other foodstuff, sweet potato has many virtues. For further exploitation of sweet potato, the separation and purification and biological activity of phenol acid in sweet potato were studied in this paper.In the study of extraction of phenol acid, Orthogonal test and single factor test were used, ethanol was used as extraction solvent. The study showed that many factors influenced extraction process, including concentration of ethanol, temperature, time, etc. Conditions that were adopted in this experiment: 50% ethanol; temperature 60℃;time 2h; matter: solvent 1:12;crush treatment 5 min ; extracting 3 times .There was a great deal of waste water in the starch production, in this waste water, many substances may callback and utilize. Extracting phenol acid from starch waste water using resin was studied. The Result showed that adsorption ratio and desorption ratio were more than 85%, and pure degree of phenol acid was 26.55%. Phenol species were a kind of compound which has"phenol"structure. It exists widely in plant. Because of its strong reactivity of hydroxide replace and capability of eliminating free radical, it has good anti-oxidation activity. Phenol acid of sweet potato can eliminate O2.- and˙OH ,which ratio related with concentration of phenol acid . It can provide protect function for hemolysis process of red cell aroused by H2O2.when the concentration of phenol acid of sweet potato was 90mg/ml,the control ratio was 28.41% .This result showed that it can restrain the happenning of hemolysis and protect red cell membrane.SOD is an important anti-oxidation enzyme. After phenol acid was fed to mice, SOD quantity of blood serum of mice has increased evidently. In the skin test, SOD quantity increased evidently, too.
Keywords/Search Tags:sweet potato, phenol, acid resin, anti-oxidant
PDF Full Text Request
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