Synthesis And Recognition Property Study Of Sulfa-drugs Molecularly Imprinted Polymer

In this thesis, progress on synthetic method or technology of Molecular imprinting polymers(MIPS) was reviewed. As a kind of artificial molecular recognition materials ,the applications of imprinted polymers including in Chromatogram analysis and separate,SolidPhaseExtraction, emulational transducer and so on which were summarized in detail . Analytic technique of iatric ort was introduced briefly. Consequently ,the profound impact with utilizing molecular imprinting technology on separating and mensurating sulfa-drugs in foodstuff and pharmacy industry were pointed out.The kernel of the thesis includes three aspects as following:The first of all is the synthesis of molecularly imprinted polymers(MIPs). Three methods are used to prepare molecular imprinted polymers,which include conventional bulk technique or polymerization with ultraviolet initiated in low temperature and polymerization with silica-gel as sacrificial materials. In the polymerization procedure , Sulfamethazine(SM2)and Sulfisoxazole(SIZ) were used as target template molecules, methacrylic acid(MAA) and 4-vinylpyridine(4-VP) and acrylamide(AA) were selected as functional monomers, ethylene glycol dimethacrylate as crosslinker, azobisisobutyronitrile was used as initator. Factors influencing polymerization were discussed, such as synthetic methods, selectivity of functional monomer, menstruum dosage , initator condition and so on. The investigation indicated in the optimal conditions as follows: acetonitrile dosage=15ml, ethylene glycol dimethacrylate dosage=20mmol, azobisisobutyronitrile dosage=120mg.Secondly,UV spectrophotometry was adopted to research the combination action on SM₂ and SIZ with MAA and 4-Vp.The combination action of functional monomer with template molecules were produced in the results. Then,the prediction imprinting principle of SM₂ and SIZ were given. The diagrams of infrared spectrum were analysed in MAA and 4-Vp and IMPs of SM₂ (SIZ). It was discoved that the absorbability peak of C=C function group in the IMPs were turn to be very low ,but the function group of carboxylic acid and pyridyl in the IMPs were no evidence change, and the condition was created in the molecular recognition of MIPs. Thermal Analysis was adopted to research IMPs ,in which ,a better thermal stability and its decompose temperature were showed in the results.Lastly, Adsorption isotherms ,adsorption static adsorption ability of MIPs for Sulfamethazine and Sulfisoxazole were measured by Ultraviolet (UV) absorptionmeasure technology. Polymer properties, which were influenced by different functional monomer, were investigated by static adsorption experiments. It was showed by Scatchard analysis :(1) polymerization with ultraviolet initiated in low temperature and polymerization with silica-gel as sacrificial materials were better than recognition. (2) Although SM₂ sand SIZ have similar structures,the interactions between them and the monomers were different. Molecular imprinting polymers had higher affinity and selectivity than NMIPS were showed in the results. Sulfamethazine-imprinted polymers using methacrylic acid as monomer had high affinity and selectivity to its template due to the ionic interaction between methazine and MAA. The static adsorption modulus was 282.3mg/ml and separate factor was 3.9. Whereas Sulfisoxazole imprinted polymer using 4-vinylpyridine as monomer could be recognition its template due to the hydrogen interaction between template and monomer . The static adsorption modulus was 241.5mg/ml and separate factor was3.74.