The Analysis Of Nateglinide And Aminoantipyrine With Molecular Imprinting And Related Technology

Molecular imprinting polymer (MIP) has draw increasing attention in recent years. Because of its higher molecular recognition ability and stability than that of biological molecules. MIP has been used in a wide rang of applications, such as chiral separartion, solid phase extraction, sensor, immuno-analysis and has become an effective strategy to prepare stationary phase of HPLC having a specific molecular recognition. In this thesis, four chapters are consisted, the technological and development of MIP, the application of MIP in Pharmaceuticals, the chiral separation of Nateglinide and the determination of degree of stripping of Nateglinide by reverse phase high performance liquid chromatography.Chapter one: it is an introduction of the basic knowledge of molecular imprinting and its application in pharmaceutical analysis.Chapter two: A synthetic polymer selector for aminoantipyrine is prepared by molecular imprinting technology. Methacrylic acid and ethylene glycol dimethacrylate are copolymerised in the presence of the template aminoantipyrine. The template is extracted from the polymer leaving specific recognition sites, complementary to the template. The polymer is utilized as a stationary phase in HPLC. The mixture of the two close analogues, aminoantipyrine and aminopyrine, can be baseline separated when the mobile solution is composed of methanol: isopropanol = 2: 8. When the concentration of isopropanol is 100%, only aminopyrine is eluted and the aminoantipyrine is completely reserved by the column.Chapter three: A high-performance liquid chromatographic method was developed for separation of a new anti-diabetic agent, N-(trans-4-isopropylcyclohexylcarbonyl)--D-phenyl- alanine, and its L-enantiomer. The separtion was performed on a Sumichiral OA-3300 column. Optimized mobile phase was 0.025 mol/L ammoniumacetate in methanol solution. UV detection was at 210nm. Baseline chiral separation was obtained within 12 minutes. The detection limits are 80 pg for D-enantiomer and 120 pg for the L-enantiomer. RSD of the method was below l%(n=5).Chapter four: A method of determination of degree of stripping(%) of Nateglinide by HPLC was developed. The chromatography conditions were as follows: column, JASCO-ODS; mobile phase, acetonitrile -methanol - NH4H2PO4(0.1 mol/1) =5:2:4(adjusted to pH 3.0 with phosphoric acid ), flow rate, 1 mL/min; detectionwavelength, 210 nm; temperature, room temperature. The degree of stripping(%) of Nateglinide was 99.9% by this method in 30 minutes.