| Liposome is a new drug delivery.It has been widely used in medical area.Liposome has many chracters:longest effect,highest effect,lowest toxiity,lowest side effect.Forfenicol is one of new pharmaceutical on the market.It is used treating animal disease.Our objection is to study the stable preparation of forfenicol-liposome.In the paper we also study the pharmcy and quality evalution of forfenicol-liposome. 1 Select the method ,the technology and prescription.We use the stability ,recovery ratio entrapment efficiency as the estimating indexes evaluating freeze-thawing method,reversed-phased method,improved reversed-phase method ,improved emulsion method,emulsion method,thin film method.The result showed that improved reversed-phase method posess the best entrapment efficiency and better stability in all of the methods. we evaluate the technology of preparation .The result showed that when the temprature of water is 40℃and the freeze-thawing times exceed of three,we can gain optimum preparation.We estimate the factor of prescription.The result showed that when the PC ∶C h is 6 ∶1,the F ∶PC is 0.7∶1 ,pH is 6.5.We can gain better encapsulation and better stability forfenicol-liposome.Its entrapment efficiency is 76.89±0.278% and the recovery ratio has not change. 2 Quality envalution of forfenicol-liposome.We estimate quality envalution according form,partical size ,stability, release property in vitro and leakage.The liposome is milk and not impurity in our eyes.The structure of forfenicol liposome was abserved with trassmission electron microscope is like a ball or like a vesicle. The mean particle size of forfenicol is 159.61nm.The 95 percent are lower than 259.9nm.5 percent are lower than 90nm.The result show a botter normal distribution. liposome can storage 60 days in 4℃. Absorption rate constant of forfenicol-liposome in vivo is 98.5% in 55 hour, the absorption rate constant of forfenicol is 99.6% in 5 hour. 3 Pharmacy study on forfenicol-liposome.We study the determination of minimal inhibitory concentrations(MICs) of fufenicol-liposome,forfenicol and soluble forfenicol and therapeutic trials with these three drugs following oral administration.In vitro inhibitory tests showed that the MICs of forfenicol-liposome on P.multocida,Staphylococcus. Aureus,Actinobacillus.pleuropneumoniae, Escherichia.coli were 1μg/ml,1μg/ml,0.25μg/ml,0.25μg/ml,2μg/ml .Inhibitory concentration is 1/4,1/4,1/8,1/4,1/4 of forfenicol and is 1/2,1/4,1/4,1/2,1/1 of soluble forfenicol . In the treatment of chickens infected with p.multocida were more effective than other drugs. The protection rate and remission rate high,medium, low of forfenicol-liposome were 100% and the cure rate were 100%,100%,88% respectively, we make chi-square for the protection rate and remission rate .The result revealed there were significant difference between forfenicol liposome and positive drug control group(P<0.05). There were no significant difference between high dosage and medium dosage(p>0.05).In the trial the weight rate of chickens were 83.9%,78.5%,72.9% respectively.there were significant difference between positive control group(P<0.05)。...
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