A Study Of The Mycotoxins In Grains

Grain samples, including maize, wheat and cereal, were sampled, inspected main fungis of harmness for grains by culturing, and tested 6 mycotoxins content of samples, including AFB, ZON, F, OTA, T-2, DON. We had a research for ZON testing method on maize. We had also a study of ozone degrading aflatoxins. The results show that: Fusarium is main hazard fungi in foretime storage, including Fusarium graminearum Schw., F.moniliforme. Aspergillius and Penicillium are main harmness fungi during storage. For immunity affinity column fluorometer method, limit of detection is O.Olppm, linearity r is 0.9996, percentage recovery is 96%, and coefficient variance is 2.9. The range of ZON: 0.11⁴.20ppm of 38 feeds, 0.04⁴.40ppm of 91 feed stuffs. For mycotoxins content survey, the mycotoxin content of stored cereal samples(n=31), wheat samples (n=31) and maize samples(n=25) were tested by the immunoaffinity column testing system in china. Results indicate that AFB (90.32%) and OTA (87.10%) have higher positive rate than and T-2 (35.48%) and DON (3.22%). As the safety level of cereal, T-2 (29.03%) exceeds OTA (3.22%) and DON (3.22%) and AFB (0) on rate of exceeding content limit of mycotoxin. The results showed that mycotoxins contamination was at large and low level. The wheat samples were in safety. The immunoaffinity column testing system in china tested the mycotoxin content of 25 storage maize samples. Results indicate that ZON (100%) and F (96%) have higher positive rate than AFB (52%) and T-2 toxin (16%) and DON (20%). Cereal samples contaminated AFB₁ were treated with ozone at 20⁵0ppm. The results indicate that ozone can degrade AFB₁ in cereal efficaciously. The degraded rate of AFB₁ is over 90% by using ozone gas for 9 hours in the most cereals. It is the most effective in rice.