Study On The Capillary Electrophoresis Of Some Tumor Markers And Anticarcinogens

Tumor, especially malignant tumor is one of the gravest threats to human beings. In this thesis, as a hot spot of analytical chemistry, capillary electrophoresis was chosen to study the tumor markers and some anticarcinogens. In part 1, the general situation of tumor markers and the progress of determination method and nucleotide were reviewed. In part 2, some modern electrochemical techniques, such as LSV, CV and DPV were used for researching the behavior of 7-methylguanosine. It provided the foundation for the following study by capillary electrophoresis with amperometric detection (CZE-AD). Their electrochemical characteristics at the surface of glassy carbon electrode have also been studied. The method was applied to the assay of 7-methylguanosine in imitative human serum and urine with satisfactory results. To investigate the curative effect of anticarcinogens, a simple, quick and sensitive method was employed for determination of 7-methylguanosine and Mitomycin by CZE-AD (in part 3). Under the optimum conditions, the current response was liner over about two orders of magnitude with detection limits (S/N =3) 0.050 and 0.025 μg/mL for 7-Methlguanosine and Mitomycine C. With the purpose of studying the toxicity of anticancer drugs that will inflict damage to human being, in part 4, a CZE-AD method was developed and applied for the separation and determination of Mitomycin, Fluoroplex, 1-Methylguanosine and Guanosine simultaneously. The conditions for separation and detection of a mixture of four analytes have been optimized, and the results showed that the investigated compounds could be successfully separated within 8 min. The method has been employed for the determination of Mitomycin, Fluoroplex, 1-Methylguanosine and Guanosine in imitative human serum, urine and plasma. Aim at the fact that combined treatment is widely used in the field of clinic. In part 5, the seven anticarcinogens were studied by used capillary electrophoresis with UV detection. Under the optimal condition, the seven anticarcinogens were baseline separated in 20 mmol/L borate buffer at pH 9.2, in less than 9 min. The proposed method has been successfully applied to analyze the imitative urine and plasma samples with satisfactory assay results. The recoveries were found to be in the range of 90.0~109.2%. In part 6, Perphenazine and Fluphenazine are determinated using CZE/end-column amperometric detection. The proposed method has been successfully applied to determine the content of Perphenazine and Fluphenazine in Perphenazine tablet, Fluphenazine tablet with satisfactory results.