Applications Of Flow Injection Chemiluminescent Techiques In The Antibacterial Pharmaceuticals

Chemiluminescence (CL) analysis offers high sensitivity , wide linear range and simple instrument. When coupled with flow-injection analysis (FIA), the CL-based FIA methods provide cheap , rapid , simple and reproducible means of detection, and therefore,have been widely used in molecular biologic,biological engineering,medicne, environmental chemistry . The thesis consist of two parts .The first is a brief review commenting the development of chemiluminescence application in pharmaceutical analysis .The other is research reports.The research reports mainly comprise the following eight parts. 1) Chemiluminescent system of amoxicillin-potassium permanganate-hydrochloric acidThe reaction between and potassium permanganate in the sulfuric acid medium gave intensive chemiluminescence.A new analytical method for determination of amoxicillin was proposed. 2 ) An investigation on the chemiluminescence reaction of amoxillin-formaldeh-yde-potassium permangate systemThe chemiluminescence reaction of amoxicillin-formaldehyde-potassium permang-nate system was examined by flow injection technique.A new method for the determination of amoxicillin was developed .3) Flow injection analysis of amoxicillin with inhibited chemiluminescence detectionA new flow injection chemiluminescence method for the determination of amoxillin was described.It was found that amoxicillin inhibited chemiluminescence from luminol-H2O2-Co2+ reaction. On this basis, a f low injection procedure combined with inhibited chemiluminescence detection was developed for the determination of amoxicllin.4) Study and application on chemiluminescent system of degradation product of amoxicillinA new flow-injection chemiluminescence (CL) method for determination of amoxicillin was described .The method was based on CL reaction of the product degraded from amoxicillin in sodium hydroxide medium with Ce(â…£) in acidic condition sensitized by rhodamine6G.5) Chemiluminescence determination of ampicillin by flow injection analysisA novel flow injection the chemiluminescence(CL) method for the determination of ampicillin was described . It was based on CL reaction of the product degraded from ampicillin in sodium hydroxide medium with potassium permanganate . The CL reactionwas sensitized by formaldehyde in acidic solution .6) Determination of lomefloxacin by Tb3+ sensitized chemiluminescenceBased on the Chemiluminescence reaction of Ce(â…£)-H2SO3- LMLX can be sensitized by Tb3+,a simple rapid high sensitive and high selective chemiluminescence method for the determination of LMLX was developed.7) Flow injection analysis of tannic acid with inhibited chemiluminescent detectionLomefloxacin aspartate was found to inhibit chemiluminescence of the luminol-H2O2 reaction catalyzed by Co2+. A flow injection method had been developed for the determination of lomefloxacin aspartate based on the inhibition of the chemiluminescence of the luminol-H2O2-Co2+ system by lomefloxacin aspartate .