Study On Extraction & Isolation And Activity Of Polyphenol In Apple

In order to making use of apple pomace enough and developing the plant resources of proanthocyanidins, Apple pomace is used as raw material mainly to establish the method of extraction and separation proanthocyanidins. Meanwhile, total phenolics and proanthocyanidins in different part and different mature degree in apple are determined. In activity research,use polyphenol of apple pomace to contrast with usual antioxidant to determine the effects on antioxidation to fats. Use proanthocyanidins of apple pomace to contrast with usual scavenger to test the clearance of free radical. All of these had provided scientific basis for further research on food industry, pharmacy, cosmetics field.1. The determination method of total phenolics and proanthocyanidins content in apple are establiahed. The order of total phenolics content is: unmmature apple>peel of mature apple > dry pomace > pulp; the order of proanthocyanidins content is: peel of mature apple >dry pomace> pulp >unmature apple.2. Using dry apple pomace as raw material, the optimal technique parameter of extracting TF and proanthocyanidins is searched by orthogonal design : 10 times liquid of 60% acetone at 40 °C withdraw 3 times, 4 hours each time.3. Using polyamide silica and solvent extraction to isolate PC are studied. The result is : the former withdraws rate is 0.0225%, the pure degree is 43.95%;The latter withdraws rate is 0.0342%, its pure degree is 34.2%.These shows that although the former withdraw rate is lower but its purty is higher.this is good for the PC further seperation.4. The antioxidative activity of apple polyphenol are researched, it expressed the order of antioxidation is: dry pomace > peel of mature apple > unmmature apple >pulp.5. Contrast with other antioxidant ,the activity of antioxidation is: TBHQ > the polyphenol of dry pomace > the tea polyphenol > BHT= VE6. The process of antioxidation exsits the equilibrium density 0.08%, lower than this density, helps to oxidize; higher than density expresses to help to antioxidize.7. In the process of antioxidation, the effect of antioxidation would be increase if pomacce polyphenol match Vc uses together.8. The scavenging of the free radicals of O2- : The relation of proanthocyanidinsquantity and effect is remarkable ,SC50 is 6 ug/ ml.9. The scavenging of the free radicals of · OH : The relation of proanthocyanidins quantity and effect have certainly, SC50 is 0.05 mg/ ml.