Study On Extract Of Apple Polyphenol And Antioxidation Effect

In this paper,the content of polyphenols in apple pomace was measured and processing conditions of polyphenols in apple pomace were optimized with mono-factor analysis and orthogonal test.The free radical clearing effect of polyphenol extraction in apple pomace was studied by using colorimentry method.Main active contents in polyphenols was identified by using HPLC method.At the same time ,changment of polyphenol concentration and quality were studied in course of ferment,the results were showed as follow: 1.The optimal conditions to identify polyphenol in apple pomace as the follows,in room temperature,add some pattern solution; 1ml Folin-phenol developer; 5ml1mol/L sodium carbonate solution and constant volume to 10ml with distilled water,mix the solution and placed in black place for 1 hour,then identified using colorimentry method. 2.Mono-factor analysis and orthogonal test were used and the optimal conditions were identified as the follows,dryness apple pomace as material ;the percent of methanol as 70%;the power of ultrasonic as 225W;the period of ultrasonic treatment as 20 minutes;the ratio of liquid to solid as 20;the number of extraction as 2 times.In this condition, content of polyphenol was170.5mg/100g. 3.Polyphenol extracts in apple pomace showed strong clear effect on superoxidized anionic radical,hydroxyl radical and lipid radical and strong inhibitory effect on oil.In methanol,ethanol and acetone extracts,methanol extract showed stongest clear effect on radical and acetone extract showed strongest inhibitory effect on oil.Clear effect on superoxidized anionic radical and hydroxyl radical of three extrations was stronger than BHT. 4.Mine avtive contents in polyphenols was identified by using HPLC method and have 8 kind contents. 5.The content of polyphenol in apple pomace decreased in course of ferment and increased 45 days later, but it still under flash apple pomace,s.In course of ferment, The changment of antioxidation of apple pomace was same to content changment of polyphenol.The content of polyphenol increased in leach solution,so apple vinegar have health protection action.