| Food pigment, an important integrant of food additives, has been widely appliedto the food industry to improve the colour and lustre of food and people's enjoymentof beauty. It has also been widely used in medicine, cosmetics and animal feed. Foodpigment is generally divided into natural and synthetic food pigment. Nowadays withthe development of toxicology and analysis techniques, the harm of synthetic pigmentto human health is becoming more and more noticeable, resulting in the prohibitionon many synthetic pigments in almost every country. Therefore, now there is a newtrend in the word's development of food additives industry, using non-toxic andharmless natural material to extract food pigment.Jasminum Nudiflorum Linde is a common flowery plant in the north and middlepart of our country, having a long flower season and blooming in bright yellow. Henceit is a comparatively ideal natural source of yellow pigment. However, so far there hasbeen no report on the development of this natural source. The purpose of this thesis isto exploit and study this natural resource, analyzing its laboratory extractiontechnology condition, stability, component, safety and antioxidant activity, thusproviding some experimental basis for developing a new natural plant pigment. Thisthesis is composed of seven parts: an introduction to the study on natural plantpigments, the choice of the extraction technology condition of yellow pigment fromJasminum Nudiflorum Linde, the proximate analysis, the study of physio-chemicalcharacteristics, the assessment of safety, the study of antioxidant activity of thisyellow pigment and a conclusion.Chapter one provides an introduction to the study on the development of someplant pigments both at home and abroad, bringing up the topic of this thesis. ChapterTwo presents a systematic study on the yellow pigments' laboratory extractioncondition through orthogonal experiments and mono-factor experiments. The resultshows that the optimum extraction condition is when Jasminum Nudiflorum Linde isdigested in 95%ethanol with ph7 and 60℃ for one hour, the feed liquid being 1:40(g:ml). The extraction rate approximates 3.8%. Chapter three makes an analysis of thebasic components of the yellow pigment from Jasminum Nudiflorum Linde throughthin layer chrornatography, chemical reaction, UV-visible absorption spectrum,IRspectrum methods and HPLC. The outcome indicates the petal of JasminumNudiflorum Linde has at least three kinds of components. One is the Rutin. The othertwo are carotenoids consisting of double bond, carbonyl and hydroxyl. Chapter fourmakes an analysis of the antioxidant activity of the yellow pigment from JasminumNudiflorum Linde by FRAP and DPPH method respectively, making an comparisonwith Vc and BHT. The outcome indicates that the total antioxidant activity of theyellow pigment from Jasminum Nudiflorum Linde is 23% more than that of BHT and22% more than that of Vc. Its capacity of clearing the free radical is 69% more thanthat of BHT and 55% more than that of Vc. The yellow pigment from JasminumNudiflorum Linde effectively inhibited Hydroxyl radical and Chemical oxygendemand was sdudied by using Fluorescent spectrophotometry andChemiluminescence. The results showed the eptal may elimination effect on O2﹣﹒and ·OH . The inhibiting effects O2﹣﹒more than·OH . The hemolysis effect onerythrocytes of pigment was studied. The present study showed the pigment may beinhinbited erythrocyte hemolysis. Chapter Five conducts a preliminary test on thesafety of this pigment. Through LD50 and Ames tests the yellow pigment fromJasminum Nudiflorum Linde has no toxicity when its chrominance is E1 1c %m=7.5, theresult from the Ames test being negative. Chapter Six gives an analysis of thephysio-chemical characteristics of yellow pigment from Jasminum Nudiflorum Linde.It is liposoluble and the maximum absorption wave of its 95%ethanol extractive is435nm. The pigment solution is relatively stable when ph value is 3~11. The pigmentis not lightfast and barely affected under 80℃ for two hours' duration of heat.Moreover, it is not quite affected by Na+, K+, Ca++,Mg++, Zn++, Fe3+, Cu++,Cl-ion, andreducer Vc and Na2so3 either, but Al3+ and oxidizer H2O2 do affect it.In conclusion, with its simple extraction technology, safety and antioxidativecapacity, the yellow pigment from Jasminum Nudiflorum Linde is a source of plantpigment worth development.
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