The Study On The Extraction And Antioxidative Effect Of The Pigment From Wild Rose

The Wild Rose distributes extensive and it's resource is plentiful. The pigment from the XinJiang HeTian wild-rose was studied in this paper, With species of extraction reagent, chroma of extraction reagent, extraction temperature, extraction time and proportion of reagent and solvent investigated in the single factor test and orthogonal test (L933 ).At the same time ,the optimum reagent of the wild rose and optimum times of extraction were studied. The result indicated that: using 5%citric acid, under 50oC, let the ratio of the solid and the liquid be 1:30, picking-up 4 hours and extracted two times are the optimum extraction condition of the water-soluble pigment from wild rose.The influence factor of stability of the pigment including photostability, themostability, resistance to oxido-reduation ,the effect of Ph value, food additives ,metal ion, the way of filtrate and deposit on the pigment were examined. Also, The result indicated that, The rose pigment is soluble in actic(pH=1-3)and water polar solvents easily, but not in non-polar organic solvents. It appears purplish red and transparent in the solution, and is stable to heat and sunlight.Most food additives and most metal ions had no effects on the pigment except that Fe2+ and Fe3+ had severe undesirable effect.Two components were isolated thought column chromatography They are identified preliminary on the basis of PC, GC, UV, chemical methods and comparison with data in the literatures. Among them, component I was purer anthocyanins, they were peonidin-3– glycoside and delphinidin -3- glycoside-rhamnoside. ComponentⅡwas inferred to be flavonoids and comprised unknown components.At the end, researched the antioxidant activities and the stability in the liquid drinks of the pigment from wild rose. The results indicated that the pigment possess antioxidant activities.in lipid. At the pigment concentration of 0.03% and 0.02% exhibits the greatest antioxidant effect for colza oil and lard. The cooperative effects were showed when the pigment was mixed with citric acid and ascorbicespecially ascorbic.