The Studies Of The Interaction Between Organic Small Molecules And Biomacromolecules

Recently, with the appearance of organic waste and the improvement of people's environmental consciousness, more and more people are interesting in the nature source and chemistry of all kinds of hazardous organic wastes and the harm approach to mankind. Hazardous organic materials and waste management is a topic that touches all lives. Every day people encounter hazardous materials (chemicals) or hazardous waste in their home, school or place of business. In order to properly manage hazardous organic waste, it is important to consider the entire life cycle, which include when the waste was a useful chemical or hazardous material and the reaction mechanism between organic small molecules and biomacromolecules. Moreover, the study of reaction mechanism between organic small molecules and biomacromolecules has very import meaning for finding new drugs, curing diseases form gene levels and studying the reaction mechanism, so it is always scholar's interesting and attentional subject. So we select some typical organic small molecules and study the reaction mechanism between organic small molecules and proteins, DNA based on the newly developmental resonance light scattering technique. The following are the main investigative systems:1. The Interaction of Isamin Blue with BSAAt pH 3.78, the static interaction of IB with BSA occurs and results in greatly enhanced resonance light scattering (RLS) signals characterized by a peak at 367.4 nm. It was found that the enhanced RLS intensity (ΔI_RLS) at 367.4 nm is proportional to the concentration of BSA, and thus a new method for trace BSA analysis could be established accordingly. The limit of determination (3σ) is 2.34 ng/mL when 1.5 × 10...