Novel Fluorescence Chemical Sensors Preparation And Their Application In The Environmental Analysis

One of the most important aspects of fluorescence chemical sensor research is searching new fluorescence carriers with high sensitivity, good selectivity, excellent reversibility and stability. At the same time, covalent immobilization is the best method for membrane preparation because the leaching of fluorophores from the sensing membrane was effectively hindered. Therefore, the author of present thesis paid attention to naphthalimide, fluorecein and Schiff bases derivatives. And they were synthesized and copolymerized under UV irradiation on the silanized glass and the sensors prepared were studied.This thesis is mainly centered on the following research aspects: Several new fluorescence carriers were designed and synthesized. With the terminal double bonds after chemical modification, they were co-polymerized with other monomers and immobilized on the modified glass surface. The sensors prepared could be used in the pH determination or some organic compounds assay. Details are presented in the thesis as follows:1. N-allyl-4-piperazinyl-1,8-naphthalimide(APN) (a pH-sensitive fluorophore) and rhodamine Rh-Al (a pH–insensitive fluorophore) were used to develop a ratiometric fluorescence pH sensor. The APN and rhodamine Rh-Al were co-polymerized with other monomers on silanized glass surface for pH sensing. The stability and lifetime of the sensor were satisfying. The sensor had rapid responses and reversible over the pH range of 5.0 to 8.0.2. Allyl eosin Y (AEY) (a pH-sensitive fluorophore) and N-(2-methacryloxyethyl) benzo[k,l]thioxanthene-3,4-dicarboximide (MBTD) (a pH-insensitive fluorophore) were prepared. They were photo-copolymerized on the silanized glass surface with the terminal double bonds. The leaching of fluorophores from the sensing membrane was hindered by covalent immobilization It can be used for the determination of pH in the range of pH 2.50-5.10, without interference of most commonly co-existing inorganic ions and some organic compounds.3. A fluorescence sensor for cetyl trimethyl ammonium bromide (CTMAB) has been developed. It is based on fluorescence quenching of allyl eosin Y. The sensing principle of the proposed sensor for CTMAB is discussed in detail. CTMAB can be determined from 5.0×10?5 to 6.0×10?3 mol l?1. The detection limit is 1.4×10?8 mol l?1.4. A new fluorescent chemical sensor for the determination of promethazine...