Research On Extracting Process Of Effective Constituent Pueraria And Its Relieving Alcoholism Function

Pueraria is an important constituent for drag and food since it has a lot of nutriments and particular medicative effects and has been widely used. As the main active component of Pueraria, Puerarin has been paid more and more attention and known for its medicative health protection function and clinic cure effect.The extraction rate of sum pueraria flavone and puerarin have been used for indication, we integrate survey the effect of factors in process on two extraction craftwork; and analysed by two factors integrative grade way; with the orthogonal test, the optimal operarion parameters of extraction process were chosen; the technique and character have been compared between two distilling procedures, and reference would be offered to industrial extraction of isoflavone from Puerarin lobata. The mice was selected to experiment object and relieving alcoholism function was studied by extracting solution from Pueraria.The effect on extraction rate from Pueraria was studied by factors including granularity,kind of solvent,amount,temperature,time and times of extraction by single factors in experiment. The extraction rate of sum Pueraria isoflavone and Puerarin have been used for indication, the optimal operarion parameters of solvent reflux extraction process were chosen by the orthogonal test: with the granularity fixed, solvent concentraction is 80% alcohol, the rate of solid to liquid is 1:30, refluxing extracted 2h in 70℃water bath.Effect of concentraction,time,temperature on extraction rate was studied mostly in ultrasonic extraction research; with the extraction rate of Pueraria isoflavone and Puerarin been index mark, the optimal operarion parameters of ultrasonic extraction process were chosen by the orthogonal test. The optimal operarion parameters: with the granularity and amount fixed, solvent concentraction is 70% alcohol, 30min in 70℃ultrasonic bath.The extraction rate have been compared between two optimizing program of extraction processing, the result indicate: extraction with ultrasonic assistant could reach upper extraction rate in short time, compared to the solvent reflex method, extraction with ultrasonic can increase the rate of sum flavone extraction 3.13%, increase the rate of puerarin 5.96%; and it need fewer solvent.so the extraction with ultrasonic can save the energy and increase efficiency.Through studying on relieving alcoholism function by Puerarin, we can know that: it can reduce the drunk time of the mice into which filled the puerarin befor drink, compared to blank control, the time from normal to drunkenness prolonged 65.96% relatively, and the time sober up...