Study On Magnifying Technology Of Puerarin's Extraction And Separation

Puerarin is a kind of resource for drug and food since it can provide people with a lot of nutriments and particular medicative effects. It has many uses. As the main active component of pueraria , puerarin is paid more and more attention increasingly for its good therapeutic effects to cardiovascular diseases, the pure product is very popular in international market.In this paper, the technology route of puerarin's extraction and separation is designed rational1y.Two key steps such as solvent extraction and rnaroreticular resin absorption and separation are studied. At the same time, the optimal operation parameters are choosed and made sure through magnifying experiments. They can provide people with necessary references for the technology-designing of puerarin s industrial production.The experimental results indicate that the sort of the extracting solvent, pH of the original solution, the sort of salt and the sort of maroreticular resin, the dosage of it(the ratio of dry extract to resin),pH of the original solution, the sort of the eluent, the velocity of flow of it are respectively the main factors which influence the purity and obtaining ratio in two steps. The optimal working parameters in solvent extraction are listed as follows:the extracting solvent is 1-butanol;the pH of the original solution is 4~5;the salt is NHiOAc. Those in maroret icular resin absorption and separation are listed as follows:the maroret icular resin AB-8;the ratio of dry extract to resin is 1:25; the pHof the original solution is 5;the eluent is 30% ethanol; the velocity of flow is 2.5ml/min and applying gradient elution to this experiment.Using these operation parameters, a higher purity and obtaining ratio and a lower energy consumption, a easier operation can be obtained in puerarin' production.