Extracting And Purification Of Lutein From Marigold Flower

Lutein, a naturally occurring carotenoid, is widely distributed in fruits and vegetables and is particularly concentrated in the Tagetes erecta flower.Marigold flowers are also the most important source of lutein for application in the food industry. As early as in the above century 80's intermediate stages, the western medicine researcher contains on the discovery plant. Naturally lutein is one kind of performance outstanding oxidation inhibitor. Lutein increases one quota to food in, may prevent the human body because the organ senilly to cause a series of diseases. The massive epidemiology evidence indicates, lutein has the protective function to the vision, and has effect and so on prevention cataract, arteriosclerosis, enhancement immunity, specially has, delays aspect and so on cancer development in the prevention cancer to play emphatically role, lutein is one hot spot on ingredient research of international function food in the present.This topic used the organic solvent extraction method withdraws lutein, and to withdrew in the process the correlation influence factor carries on the inspection, for example the volume compared to, withdrew the time, the material fluid compared to and so on, finally determined the best craft route of extraction lutein from marigold flower, had the promoted value, might for marigold flower promoted planting provided the theory basis. This topic takes the xanthophylls as the raw material, designs one. kind of separation purification craft. In this research, lutein is separated with other kind of households radish element under the following chromatograph condition: VP-ODS C₁₈ column(150×4.6mm,Φ4μm); Flows: V(CH₂Cl₂): V(CH₃OH): V(CH₃CN): V(H₂O) = 32:29:38:1; Temperature: Room temperature; Speed of flow: 0.5ml/min; Examination wave length: 454nm;And carried on qualitative and the quota examination to lutein, we used two examinations methods: Spectrophotometer law and liquid phase chromatography. The product purity achieves above 90%. In addition, lutein takes...