Study On Chiral Separation Of Cetirizine Enantiomers By Capillary Eletrophoresis In The Media Of Non-cyclic Saccharides

Compared with High Performance Liquid Chromatograhpy(HPLC), chiral separation by capillary electrophoresis(CE) has the following advantages: 1) higher separation efficiency, 2) without chiral column, 3) wider application ranges because it be used for the separation of molecules, ions and compounds with water-solubility or oil-soluvility, 4) various separation modes, 5) less yieds of chiral selectors. The success of the chiral separation by CE mainly depends on the appropriate chiral selector(CS). Non-cyclic saccharide is a new kind of CSs which was primarily known and applied around academe, and the use of it in chiral separation by CE was limited. Therefore, amylose, dextrin, dextran, maltose, sucrose and glucose were used as the representations of Non-cyclic saccharides, and we studied their abilities for separation the enantiomers of the anti-histamine drug cetirizine as the CS by the number. The influences of the length of chain and the linkage type of these saccharides were also discussed.In the first part of the thesis, the chiral separation of cetirizine enantiomers were successfully achieved by using amylose as CS in CE. The concentration of amylose, the ionic intensity and pH of the buffer, running voltage and organic additive were reviewed, and the conditions of the experiment were optimized, too. The sequence of peaks of S -(+) - cetirizne and R - (-) - cetirizine were affirmed, and the binding intensities between two enantiomers and amylose were studied.Secondly, another sort of Non-cyclic saccharide which has shorter length of chain was used for the separation of cetirizine as the CS by CE. In the running buffer composed of 80 mmol·L^-1 phosphate containing 5.0 % (w/w) dextrin solution with pH 2.5, and at the applied voltage of 20 kV, the enantiomers of cetirizine could be well separated with the resolution of 2.0. The linear range of concentration of R - (-) - CTRZ in the quantitative analysis of cetirizine was 0.05 5.00 g·L^-1. The correlation coefficient was over 0.9990, and the RSD was less than 4.7 %. In the same time dextrin was also used in the study, for its similar structure except the kind of linkage.Thirdly, farther study was carried out by using maltose, sucrose and glocuse for the purpose of research the influence of the length of chain and the linkage type of these saccharides. The above three sorts of saccharides were used as CS for the separation of cetirizine enantiomers by CE. In the current conditions, only maltose showed the chiral separation, and we didn't find any evidence of separation of cetirizine enantiomers by...