| This paper consists of four parts.Chapter 1 In this section, advances in glycosidation modification of flavoniodswere reviewed. The significance and direction of the research were elucidated.Chapter 2 The conditions for synthesis and purification of genistein glucosidesby phase transfer catalyzed method was investigated, and the structuralcharacterization of target compound was also conducted by IR, 1H NMR, 13C NMRand MS spectra in this part.Chapter 3 The abilities of genistein and their derivatives in free radicalscavenging of·OH and·O2- and DPPH were exploited by using Fenton reaction withO-phenanthroline chromogenic reaction, pyrogallol autoxidation method and UV-visspectrophotometry, respectively. The results show that the abilities of glycosidederivatives of genistein in scavenging of the three free radicals were not obviouslyimproved compared withgenistein.Chapter 4 The interactions of genistein, genistein glucoside and genistein7,4'-di-O-β- D- glucoside with calf thymus DNA(ctDNA) were investigated in thissection by using means of UV spectra, fluorescent spectra and viscosity, respectively.The results showed that the UV spectra of genistein and its glucosides showedobviously hypochromism under the presence of ctDNA. In addition, the testedcompounds could make the fluorescence intensity of EB-DNA system decreasedsharply, and also increased the relative viscosity of ctDNA aqueous. Theexperimental results suggested that genistein and its glucosides could bind to ctDNAby combination of partly intercalation and hydrogen bonding mode.
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