| This paper consists of four parts.Chapter 1 In this section, advances in glycosidation modification of flavoniods were reviewed. The significance and direction of the research were elucidated.Chapter 2 The conditions for synthesis and purification of genistein xylosides by phase transfer catalyzed method was investigated, and the structural characterization of target compound was also conducted by IR, 1H NMR, 13C NMR and MS spectra in this part.Chapter 3 The abilities of genistein and their derivatives in free radical scavenging of·OH and·O2- and DPPH were exploited by using Fenton reaction with O-phenanthroline chromogenic reaction, pyrogallol autoxidation, methodUV-vis spectrophotometry and protective effect of Genistein and its glycoside on hydroxyl radicals mediated DNA damage, respectively. The results show that the abilities of glycoside derivatives of genistein in scavenging of the three free radicals were not obviously improved compared with genistein.And show can prevent DNA damage from hydroxyl radicals.Chapter 4 The interactions of genistein, genistein xyloside with calf thymus DNA(ctDNA) were investigated in this section by using means of UV spectra, fluorescent spectra and viscosity, respectively. The results showed that the UV spectra of genistein and its glycosides showed obviously hypochromism under the presence of ctDNA. In addition, the tested compounds could make the fluorescence intensity of EB-DNA system decreased sharply, and also increased the relative viscosity of ctDNA aqueous. The experimental results suggested that genistein and its glycosides could bind to ctDNA by combination of partly intercalation and hydrogen bonding mode.
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