Studies On The Rare-earth Doped Inorganic Luminescent Nanoparticles Applied To Immunoassay

Nanoparticales as biological fluorescence probe possess excellent optical properties, such as: high luminescence intensity, long fluorescence life time. They are also photo-chemically stable and they have broad Stokes displacement and they can not blanch by light. However, the luminescence materials prepared at present are commonly pockety in granularity, and have anomalous shape. Therefore, this dissertation systematically investigated the controlled synthesis and optical properties of lanthanide nanophosphors. They show potential application in luminescence labels. The results of this dissertation are summarized as follows:Eu³⁺-doped YVO₄ nanoparticles were prepared by a microemulsion process at room temperature. The effects of reaction time and initial pH on the morphology, size and luminescence of YVO₄: Eu³⁺ are systematically investigated. The samples have high luminescence intensity even without any calcination. They answer for the perfect nanoparticles label's request in luminescent efficiency and granularity size respect.Near-infrared luminescent materials could instead of visible luminescent materials applied to biologic label by enhancing the near-infrared luminescent materials' luminescence intensity and excitated efficiency. Therefore, we try to prepareing Ln(Ln=Y, Gd) VO₄: Er³⁺/Nd³⁺ by solid state reaction. The result illuminated that there are energy transfer from VO₄^3- tO Er³⁺/Nd³⁺. Therefore, the luminescence intensity of (Ln=Y, Gd) VO₄: Er³⁺/Nd³⁺ have been enhanced.Eu³⁺-doped LaPO₄ nanoparticles were prepared by a hydrothermal process with PEG2000 used as additive. The nanoparticles are well-dispersed and similar to sphere. They show potential application in optical sensor.Eu³⁺-doped CaWO₄ nanoparticles were prepared by a facile microemulsion-mediated hydrothermal procedure. SEM shows that the nanoparticles are similar to sphere. The products have high intensity and long-lasting phosphorescence after calcinations, and they show potential application in long-lasting phosphorescence label.