Optimizate The Craft Of Phytic Acid's Preparation And Its Pharmacological Action Research

This experiment optimized the craft condition of the phytin's extraction and making thephytic acid's craft condition, I optimized the craft condition of the phytic's extraction, andoptimized the hydrolysis phytic making the phytic acid. Studied the main factors of extractionquantity and the degree of purification of phytic. Under series of experiments, the craftcondition of the phytic's extraction, the hydrolysis phytic making the phytic acid and thepharmacological action in degreeing the blood fat and the blood sugar has been given, giveout the pharmacological action, toxicological characteristics and the effect on improvingimmunity by mice experiment.I had carrying on the extraction of phytic acid from the fuel ethyl alcohol fermentationby-product. I had get the best craft condition from the orthogonal experiment. The best craftcondition: acid concentration is 0.8ï¼…; acid pickling time is 6h; extracting temperature is 60℃; the alkali precipitating condition is lime (10ï¼…),NaHCO₃ (10ï¼…) and NaOH(1mol/L),pH is 6.5.The condition of the phytic acid' s extraction had been making from the singal factor.The vibration condition is interrupted vibration,the extracting acid is hydrochloric acid,theneutralizaing agent is mix neutralizaing agent.Hydrolisis the phytic acid, the best Speed of flow of the 732# positive ion exchangeresin is 375mL/h; the big entering quantity of the type is 0.5mL; the best decolorization of theactivated carbon is 1mL/2min, the temperature is 60℃; the temperature of the vacuumreduced pressure concentration is 50℃. Under the up process, We can get the better qualityphytic acid.Under the phytic acid quality examination, We used the nitrogen meter examined thenitrogen of the phytic acid; using the Bradford method had been analysised the proteincontent of the phytic acid; used the comparing precipitation muddy had get the sulfateradical content is 0.003ï¼…,the calcium ion content is 23ï¼…,the hard ion content is 0.0003ï¼…,chloride ion content is 0.002ï¼…,phosphorus content is 34ï¼…, and the water content in phyticacid is 12ï¼…, above conforms to the standards which in the pharmacopoeia stipulated of ourcountry.I had used the high-performance analysis the phytic acid which extrcated from the fuelethyl alcohol fermentation by-product. Elution profile of phytic acid standard and of thedistiller's grain sample. Separation of phytic acid on a Omnipac pax—100 column; using the200 mM NaOH, water—isopropanol (1:1, v/v) and water (18mΩ) as eluents, and chemically suppressed conductivity using an ASRS-I 4 mm.I had used the high fat feed established the high blood fats model and the diabetesmodel. I had used low,center and high dosage feeded the mouse, and got the phytic acid's function in reducing the TG, TC, HDL-C and blood sugar.These experiment had confirmedthe function of the phytic acid in treating the high blood fats and diabetes and diabetes' sillness complication.After 1 month, we weighed the avoirdupois of mice normally poured stomach byphytic acid, and weighed the liver, spleen, kidney, heart, lungh and recorded the index ofthe organs respectively. The result shows that the phytic acid can increase the liver quality,spleen quality and heart quality, and nothing bad effect on the other organs. The conclusion isthat the phytic acid can improve the immune of the mouse. By theswimming experiment andresistance to vhypoxia experiment, we knew that the effect of phytic acid had large functionon improving resistance to hypoxia capability and viability at anoxic condition.Though our experiment can use in the production, we can improve the optimization ofthe phytin's extraction and preparation craft of the phytic acid now. Use the abandon rawmaterials increase attachment value of the fuel ethyl alcohol fermentation by-product, makesure the fuel ethyl alcohol enterprise can revolute in a benigning type, and reslove thedemending of the enterprises all over the world. Based on the function of the phytic acidwhich of reducing the blood fats and the blood sugar. We can use the phytic acid developassistance medicine; based on the function of the phytic acid which can guarantees theliver,healthy brain and clear vision, we develop it into a new drink, make it contribute tohealthy crowd.